Osteopontin drives KRAS-mutant lung adenocarcinoma

Giopanou, Ioanna, Kanellakis, Nikolaos I, Giannou, Anastasios D, Lilis, Ioannis, Marazioti, Antonia, Spella, Magda, Papaleonidopoulos, Vassilios, Camargo Madeira Simoes, Davina, Zazara, Dimitra E, Agalioti, Theodora, Moschos, Charalampos, Magkouta, Sophia, Kalomenidis, Ioannis, Panoutsakopoulou, Vily, Lamort, Anne-Sophie, Stathopoulos, Georgios T and Psallidas, Ioannis (2020) Osteopontin drives KRAS-mutant lung adenocarcinoma. Carcinogenesis, 41 (8). pp. 1134-1144. ISSN 0143-3334

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Official URL: https://doi.org/10.1093/carcin/bgz190

Abstract

Increased expression of osteopontin (SPP1) is associated with aggressive human lung adenocarcinoma, but its function remains unknown. Our aim was to determine the role of SPP1 in smoking-induced lung adenocarcinoma. We combined mouse models of tobacco carcinogen-induced lung adenocarcinoma, of deficiency of endogenous Spp1 alleles, and of adoptive pulmonary macrophage reconstitution to map the expression of SPP1 and its receptors and determine its impact during carcinogenesis. Co-expression of Spp1 and mutant KrasG12C in benign cells was employed to investigate SPP1/KRAS interactions in oncogenesis. Finally, intratracheal adenovirus encoding Cre recombinase was delivered to LSL.KRASG12D mice lacking endogenous or overexpressing transgenic Spp1 alleles. SPP1 was overexpressed in experimental and human lung adenocarcinoma and portended poor survival. In response to two different smoke carcinogens, Spp1-deficient mice developed fewer and smaller lung adenocarcinoma with decreased cellular survival and angiogenesis. Both lung epithelial- and macrophage-secreted SPP1 drove tumor-associated inflammation, while epithelial SPP1 promoted early tumorigenesis by fostering the survival of KRAS-mutated cells. Finally, loss and overexpression of Spp1 was, respectively, protective and deleterious for mice harboring KRASG12D-driven LADC. Our data support that SPP1 is functionally involved in early stages of airway epithelial carcinogenesis driven by smoking and mutant KRAS and may present an important therapeutic target.

Item Type: Article
Uncontrolled Keywords: SPP1, KRAS, urethane, lung cancer, survival
Subjects: A300 Clinical Medicine
B100 Anatomy, Physiology and Pathology
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Elena Carlaw
Date Deposited: 10 Dec 2019 11:58
Last Modified: 19 Nov 2020 03:30
URI: http://nrl.northumbria.ac.uk/id/eprint/41704

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