Systematic Comparison of Retinal Organoid Differentiation from Human Pluripotent Stem Cells Reveals Stage Specific, Cell Line, and Methodological Differences

Mellough, Carla B., Collin, Joseph, Queen, Rachel, Hilgen, Gerrit, Dorgau, Birthe, Zerti, Darin, Felemban, Majed, White, Kathryn, Sernagor, Evelyne and Lako, Majlinda (2019) Systematic Comparison of Retinal Organoid Differentiation from Human Pluripotent Stem Cells Reveals Stage Specific, Cell Line, and Methodological Differences. Stem Cells Translational Medicine, 8 (7). pp. 694-706. ISSN 2157-6564

[img]
Preview
Text
sctm.18-0267.pdf - Published Version
Available under License Creative Commons Attribution 4.0.

Download (12MB) | Preview
Official URL: https://doi.org/10.1002/sctm.18-0267

Abstract

A major goal in the stem cell field is to generate tissues that can be utilized as a universal tool for in vitro models of development and disease, drug development, or as a resource for patients suffering from disease or injury. Great efforts are being made to differentiate human pluripotent stem cells in vitro toward retinal tissue, which is akin to native human retina in its cytoarchitecture and function, yet the numerous existing retinal induction protocols remain variable in their efficiency and do not routinely produce morphologically or functionally mature photoreceptors. Herein, we determine the impact that the method of embryoid body (EB) formation and maintenance as well as cell line background has on retinal organoid differentiation from human embryonic stem cells and human induced pluripotent stem cells. Our data indicate that cell line‐specific differences dominate the variables that underline the differentiation efficiency in the early stages of differentiation. In contrast, the EB generation method and maintenance conditions determine the later differentiation and maturation of retinal organoids. Of the latter, the mechanical method of EB generation under static conditions, accompanied by media supplementation with Y27632 for the first 48 hours of differentiation, results in the most consistent formation of laminated retinal neuroepithelium containing mature and electrophysiologically responsive photoreceptors. Collectively, our data provide substantive evidence for stage‐specific differences in the ability to give rise to laminated retinae, which is determined by cell line‐specific differences in the early stages of differentiation and EB generation/organoid maintenance methods at later stages.

Item Type: Article
Uncontrolled Keywords: Human embryonic stem cells, Induced pluripotent stem cells, Retina, Organoids, Tissue engineering
Subjects: C400 Genetics
C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Elena Carlaw
Date Deposited: 11 May 2020 16:13
Last Modified: 31 Jul 2021 18:04
URI: http://nrl.northumbria.ac.uk/id/eprint/43069

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics