Impaired Condensin Complex and Aurora B kinase underlie mitotic and chromosomal defects in hyperdiploid B-cell ALL

Menendez, Pablo, Bueno, Clara, Calvo, Maria, Camos, Mireia, Rodríguez, Rene, Ramírez, Manuel, Calasanz, Maria Jose, Rodriguez-Perales, Sandra, Perez-Iribarne, Maria del Mar, Plensa, Isabel, Den Boer, Monique L., Ballerini, Paola, Pal, Deepali, Tirados-Menéndez, Sofía, Trincado, Juan Luis, Rodríguez-González, Pablo, López-López, Carlos Manuel, Valledor, Luis, Gutierrez-Agüera, Francisco, Roca-Ho, Heleia, Granada, Isabel, Vinyoles, Meritxell and Molina, Oscar (2020) Impaired Condensin Complex and Aurora B kinase underlie mitotic and chromosomal defects in hyperdiploid B-cell ALL. Blood, 136 (3). pp. 313-327. ISSN 0006-4971

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Impaired condensin complex paper Blood 2020.pdf - Accepted Version
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Official URL: https://doi.org/10.1182/blood.2019002538

Abstract

B-cell acute lymphoblastic leukemia (B-ALL) is the most common pediatric cancer, and high-hyperdiploidy (HyperD) identifies the most common subtype of pediatric B-ALL. Despite HyperD is an initiating oncogenic event affiliated to childhood B-ALL, the mitotic and chromosomal defects associated to HyperD B-ALL (HyperD-ALL) remain poorly characterized. Here, we have used 54 primary pediatric B-ALL samples to characterize the cellular-molecular mechanisms underlying the mitotic/chromosome defects predicated to be early pathogenic contributors in HyperD-ALL. We report that HyperD-ALL blasts are low proliferative and show a delay in early mitosis at prometaphase, associated to chromosome alignment defects at the metaphase plate leading to robust chromosome segregation defects and non-modal karyotypes. Mechanistically, biochemical, functional and mass-spectrometry assays revealed that condensin complex is impaired in HyperD-ALL cells, leading to chromosome hypocondensation, loss of centromere stiffness and mis-localization of the chromosome passenger complex proteins Aurora B Kinase (AURKB) and Survivin in early mitosis. HyperD-ALL cells show chromatid cohesion defects and impaired spindle assembly checkpoint (SAC) thus undergoing mitotic slippage due to defective AURKB and impaired SAC activity, downstream of condensin complex defects. Chromosome structure/condensation defects and hyperdiploidy were reproduced in healthy CD34+ stem/progenitor cells upon inhibition of AURKB and/or SAC. Collectively, hyperdiploid B-ALL is associated to defective condensin complex, AURKB and SAC.

Item Type: Article
Subjects: A300 Clinical Medicine
B100 Anatomy, Physiology and Pathology
C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Elena Carlaw
Date Deposited: 19 May 2020 08:45
Last Modified: 15 Sep 2020 14:45
URI: http://nrl.northumbria.ac.uk/id/eprint/43182

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