Bioactive nanocomposite coatings under visible light illumination promoted surface-mediated gene delivery

Yao, Lili, Wang, Xiaozhao, Weng, Wenjian, Fu, Richard and Cheng, Kui (2020) Bioactive nanocomposite coatings under visible light illumination promoted surface-mediated gene delivery. Biomaterials Science, 8 (13). pp. 3685-3696. ISSN 2047-4830

[img] Text
Revised_Manuscript_Biomaterial_Science_0505.pdf - Accepted Version
Restricted to Repository staff only until 11 May 2021.

Download (781kB) | Request a copy
Official URL: https://doi.org/10.1039/d0bm00123f

Abstract

Gene delivery based on bioactive coatings on collagen has great potential for applications in bone repair. Meanwhile, controlled gene delivery at specific times/regions is essential for an efficient and complete bone reconstruction process. However, spatio-temporal regulation of gene release and delivery remains a great challenge. In this paper, we used visible light illumination to effectively regulate gene release and subsequent delivery into biological cells. A visible light responsive and bioactive nanocomposite coating (based on collagen/gold nanoparticles, e.g., Col/AuNPs) was prepared through hydrothermal and sol–gel processes and was used as a loading platform for complexes of enhanced green fluorescent protein and Lipofectamine2000 (LF/GFP). The results showed that the amount of immobilized LF/GFP was increased on Col/AuNPs and the release of pre-adsorbed LF/GFP was significantly enhanced in a spatio-temporal and controlled manner under visible light illumination. Moreover, the cellular intake of the released genes was improved, thus enhancing the gene expression efficiency of the cells. The mechanism of enhanced controlled gene delivery was attributed to the changes in collagen structures and rearrangement of cytoskeletal structures induced by the photothermal effect. The developed Col/AuNP composite coating is effective for both controlled surface-mediated gene delivery and gene-mediated bone repair.

Item Type: Article
Subjects: C100 Biology
C400 Genetics
C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Engineering and Environment > Mathematics, Physics and Electrical Engineering
Depositing User: Rachel Branson
Date Deposited: 26 May 2020 10:52
Last Modified: 13 Oct 2020 15:00
URI: http://nrl.northumbria.ac.uk/id/eprint/43242

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics