Biosafety of a 3D printed intraocular lens made of a poly(acrylamide co sodium acrylate) hydrogel in vitro and in vivo

Li, Jiawen, Li, Yijian, Hu, Xisu, Yu, Gong, Xu, Bin, Xu, Haiwei and Yin, Zheng Qin (2020) Biosafety of a 3D printed intraocular lens made of a poly(acrylamide co sodium acrylate) hydrogel in vitro and in vivo. International Journal of Ophthalmology. ISSN 2222-3959 (In Press)

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Abstract

The biosafety of the poly(acrylamide-co-sodium acrylate) hydrogel was first 19 analyzed in vitro using human lens epithelial cells (LECs) and the ARPE19 cell line, and a CCK-8 assay was performed to investigate alterations in cell proliferation. A thin film of a poly(acrylamide-co-sodium acrylate) hydrogel and a conventional IOL were intraocularly implanted into the eyes of New Zealand white rabbits respectively, and a sham surgery served as control group. The anterior segment photographs, intraocular pressure (IOP), blood parameters and electroretinograms were recorded. Inflammatory cytokines in the aqueous humor, such as TNF and IL-8, were examined by ELISA. Cell apoptosis of the retina was investigated by TUNEL assay, and macrophage activation was detected by immunostaining.

Item Type: Article
Uncontrolled Keywords: Cataract; Biosafety; Lens Epithelial Cells; ARPE19 Cells; Intraocular Lens; 3D 39 Printing; Poly Hydrogel; Rabbit
Subjects: B500 Ophthalmics
Department: Faculties > Engineering and Environment > Mechanical and Construction Engineering
Depositing User: John Coen
Date Deposited: 07 Aug 2020 13:47
Last Modified: 07 Aug 2020 14:00
URI: http://nrl.northumbria.ac.uk/id/eprint/44021

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