Synthesis and Initial Evaluation of a Novel Fluorophore for Selective FMDV 3C Protease Detection

Malik, Samerah, Sinclair, Alex, Ryan, Ali and Le Gresley, Adam (2020) Synthesis and Initial Evaluation of a Novel Fluorophore for Selective FMDV 3C Protease Detection. Molecules, 25 (16). p. 3599. ISSN 1420-3049

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Official URL: https://doi.org/10.3390/molecules25163599

Abstract

The development and evaluation of a Boc-AL(Boc)Q(Trt)-AMC fluorophore to detect 3C Protease, produced by Foot and Mouth Disease Virus (FMDV) is reported, with a view to a potential use as a rapid screen for FMDV infected livestock The peptide-linked conjugate fluorophore is evaluated in vitro for sensitivity, specificity, stability and rapidity and shows statistically significant increases in fluorescence when exposed to physiologically relevant concentrations of 3C Protease and selectivity when compared with other common proteases likely to be located, typically in the absence of FMDV. The stability of deprotected Boc-AL(Boc)Q(Trt)-AMC is reported as a limitation of this probe.

Item Type: Article
Uncontrolled Keywords: FMDV; 3C protease; fluorescence; veterinary testing
Subjects: C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Elena Carlaw
Date Deposited: 07 Aug 2020 15:24
Last Modified: 07 Aug 2020 15:30
URI: http://nrl.northumbria.ac.uk/id/eprint/44026

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