Experimental and Genomic Evaluation of the Oestrogen Degrading Bacterium Rhodococcus equi ATCC13557

Harthern-Flint, Sarah L., Dolfing, Jan, Mrozik, Wojciech, Meynet, Paola, Eland, Lucy E., Sim, Martin and Davenport, Russell J. (2021) Experimental and Genomic Evaluation of the Oestrogen Degrading Bacterium Rhodococcus equi ATCC13557. Frontiers in Microbiology, 12. p. 670928. ISSN 1664-302X

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Official URL: https://doi.org/10.3389/fmicb.2021.670928

Abstract

Rhodococcus equi ATCC13557 was selected as a model organism to study oestrogen degradation based on its previous ability to degrade 17α-ethinylestradiol (EE2). Biodegradation experiments revealed that R. equi ATCC13557 was unable to metabolise EE2. However, it was able to metabolise E2 with the major metabolite being E1 with no further degradation of E1. However, the conversion of E2 into E1 was incomplete, with 11.2 and 50.6% of E2 degraded in mixed (E1-E2-EE2) and E2-only conditions, respectively. Therefore, the metabolic pathway of E2 degradation by R. equi ATCC13557 may have two possible pathways. The genome of R. equi ATCC13557 was sequenced, assembled, and mapped for the first time. The genome analysis allowed the identification of genes possibly responsible for the observed biodegradation characteristics of R. equi ATCC13557. Several genes within R. equi ATCC13557 are similar, but not identical in sequence, to those identified within the genomes of other oestrogen degrading bacteria, including Pseudomonas putida strain SJTE-1 and Sphingomonas strain KC8. Homologous gene sequences coding for enzymes potentially involved in oestrogen degradation, most commonly a cytochrome P450 monooxygenase (oecB), extradiol dioxygenase (oecC), and 17β-hydroxysteroid dehydrogenase (oecA), were identified within the genome of R. equi ATCC13557. These searches also revealed a gene cluster potentially coding for enzymes involved in steroid/oestrogen degradation; 3-carboxyethylcatechol 2,3-dioxygenase, 2-hydroxymuconic semialdehyde hydrolase, 3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase, 3-(3-hydroxy-phenyl)propionate hydroxylase, cytochrome P450 monooxygenase, and 3-oxosteroid 1-dehydrogenase. Further, the searches revealed steroid hormone metabolism gene clusters from the 9, 10-seco pathway, therefore R. equi ATCC13557 also has the potential to metabolise other steroid hormones such as cholesterol.

Item Type: Article
Additional Information: Funding information: This work was supported by the EPSRC Challenging Engineering Grant (EP/I025782/1; Mitigating the risk of micropollutants in the environment). Genomic DNA extraction and sequencing was supported by the EPSRC An New Frontier in Design: The Simulation of Open Engineered Biological Systems Grant (EP/K039083/1) and performed at the Interdisciplinary Computing and Complex Biosystems research group, School of Computing, Devonshire Building, Newcastle University, Newcastle-upon-Tyne, NE1 7RU, UK by the Prof. Wipat team.
Uncontrolled Keywords: Rhodococcus equi, oestrogen, genome, degradation, genes, bacteria
Subjects: C500 Microbiology
Department: Faculties > Engineering and Environment > Mechanical and Construction Engineering
Depositing User: Elena Carlaw
Date Deposited: 16 Jul 2021 09:54
Last Modified: 16 Jul 2021 09:54
URI: http://nrl.northumbria.ac.uk/id/eprint/46689

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