Bacterial diversity in house dust: characterization of a core indoor microbiome

Entwistle, Jane, Thompson, Jonathan R., Argyraki, Ariadne, Bashton, Matthew, Bramwell, Lindsay, Crown, Matthew, Hursthouse, Andrew, Jabeen, Khadija, Marinho-Reis, Amélia P., Namdeo, Anil, Nelson, Andrew, Pearce, David, Potgieter-Vermaak, Sanja, Rasmussen, Pat E. and Wragg, Joanna (2021) Bacterial diversity in house dust: characterization of a core indoor microbiome. Frontiers in Environmental Science. ISSN 2296-665X (In Press)

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Official URL: https://doi.org/10.3389/fenvs.2021.754657

Abstract

Our indoor microbiome consists of a wide range of microbial taxa. Whilst many of these microbes are benign, some are beneficial, some harmful, yet our knowledge of the spatial heterogeneity of bacterial assemblages in our residential environment remains limited. To investigate the existence of a common core house dust bacterial microbiome we selected household vacuum dusts, collected through a citizen science approach, from homes across two bioclimatic regions (UK, Oceanic/Maritime and Greece, Mediterranean). Following the extraction of DNA from each dust sample, we targeted the bacterial 16S rRNA gene using Illumina NextSeq sequencing. PERMANOVA analysis of the microbial communities at family level grouped samples within their distinct bioclimatic region and SIMPER analysis at genus level identified the statistically significant taxa responsible for driving diversity between these groups. A ‘common to all’ core house dust microbiome consisted of Acinetobacter, Massalia, Rubellimicrobium, Sphingomonas and Staphylococcus; genera typically associated with human occupancy and common environmental sources. Additionally, a ‘unique location specific’ microbiome was identified, reflective of the bioclimatic region. The Greek dusts indicated a lower average diversity than the UK house dusts, with a high abundance of Rhizobaciacae in the Greek samples. Our study highlights citizen science as a powerful approach to access the indoor residential environment, at scale, and establishes the existence of a ‘core’ house dust microbiome independent of bioclimatic region.

Item Type: Article
Additional Information: Funding information: JE acknowledges funding from the Natural Environment Research Council (Research Grant NE/T004401/1). MB and MC acknowledge research funding from Research England’s Expanding Excellence in England (E3) Fund as part of the Hub for Biotechnology in the Built Environment. KJ acknowledges funding for her PhD research programme from Northumbria University. Open access publication fees were provide by Northumbria University.
Uncontrolled Keywords: Vacuum dust, Bacterial assemblage, citizen science, 16S, microbiome
Subjects: C500 Microbiology
F900 Others in Physical Sciences
Department: Faculties > Engineering and Environment > Geography and Environmental Sciences
Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Elena Carlaw
Date Deposited: 27 Oct 2021 12:36
Last Modified: 27 Oct 2021 12:59
URI: http://nrl.northumbria.ac.uk/id/eprint/47581

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