Nitrogen-doped carbon hollow trunk-like structure as a portable electrochemical sensor for noradrenaline detection in neuronal cells

Emran, Mohammed Y., Shenashen, Mohamed A., Elmarakbi, Ahmed, Selim, Mahmoud M. and El-Safty, Sherif A. (2022) Nitrogen-doped carbon hollow trunk-like structure as a portable electrochemical sensor for noradrenaline detection in neuronal cells. Analytica Chimica Acta, 1192. p. 339380. ISSN 0003-2670

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Official URL: https://doi.org/10.1016/j.aca.2021.339380

Abstract

To date, the production and development of portable analytical devices for environmental and healthcare applications are rapidly growing. Herein, a portable electrochemical sensor for monitoring of noradrenaline (NA) secreted from living cells using mesoporous carbon-based materials was fabricated. The modification of the interdigitated electrode array (IDA) by nitrogen-doped mesoporous carbon spheres (N-doped MCS) and nitrogen-doped carbon hollow trunk-like structure (N-doped CHT) was used to fabricate the NA sensor. The N-doped CHT surface shows multiple holes distributed with micrometer-sized open holes (1–2 μm) and nanometer-sized thin walls (∼98 nm). The N-doped CHT surface heterogeneity of wrinkled and twisted hollow trunk structures improve the diffusion pathway and the NA molecules loading. The N-doped CHT/IDA showed a highly selective assay for monitoring of NA with high sensitivity (1770 μA/μM × cm2), a low detection limit (0.005 μM), and a wide linear range (0.01–0.3 μM). The N-doped CHT/IDA monitored the NA secreted from PC12 cells under various concentrations of simulation agents (KCl). The designed N-doped CHT/IDA provides a portable NA-sensor assay with facile signaling, good stability, high biocompatibility, in-vitro assay compatibility, and good reproducibility. Therefore, the designed sensor can be used as a portable sensor for NA detection in live cells and can be matched with portable smartphones after further developments.

Item Type: Article
Additional Information: Funding Information: This work was supported by the Japan Society for the Promotion of Science (JSPS) , grant No. P19067.
Uncontrolled Keywords: Portable electrochemical sensor, Noradrenaline, In-Vitro assay, Alzheimer diseases, PC12, Porous N-doped carbon materials
Subjects: F200 Materials Science
H600 Electronic and Electrical Engineering
Department: Faculties > Engineering and Environment > Mechanical and Construction Engineering
Depositing User: John Coen
Date Deposited: 11 Jan 2022 13:05
Last Modified: 11 Jan 2022 13:15
URI: http://nrl.northumbria.ac.uk/id/eprint/48134

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