Tapered side-polished microfibre sensor for high sensitivity hCG detection

Luo, Wei, Liu, Bin, Liu, Juan, Wu, Tao, Liu, Qiang, Wang, Meng-Yu, Wan, Sheng-Peng, Yuan, Jinhui, Lu, Ping, Wang, Danling, He, Xing-Dao and Wu, Qiang (2022) Tapered side-polished microfibre sensor for high sensitivity hCG detection. IEEE Sensors Journal, 22 (8). pp. 7727-7733. ISSN 1530-437X

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Official URL: https://doi.org/10.1109/JSEN.2022.3156284

Abstract

A high sensitivity human chorionic gonadotropin (hCG) detection was conducted by a tapered side-polished (TSP) optical fiber sensor. Experimentally, the TSP fiber sensor was made by side polishing a short section of single mode fiber to a D shape structure and tapering the D shape section to a small diameter (<10 μm in the experiments). By functionalizing the primary antibody of hCG onto the TSP fiber surface, the sensor was used for detecting hCG concentration. Experimental results show that when the hCG concentration is 0.1 mIU/mL, the sensor has an average wavelength shift of 0.82 nm. The limit of detection (LoD) of the hCG is estimated 0.058 mIU/mL, assuming three times of maximum wavelength variation (3×0.15=0.45 nm) in Phosphate buffer saline (PBS) to the measurement limit. The specificity has also been tested by immersing the sensor into a mixed biomaterial solution (hCG -1 mIU/mL, pig-IgG -1 /mL, Staphylococcus aureus -6×105 CFU/mL and Escherichia coli -2.5×105 CFU/mL). The result showed that the TSP optical fiber sensor has excellent specificity. The biosensor has potential application in clinical/medical diagnostics, human health, environmental quality and food safety monitoring.

Item Type: Article
Additional Information: Funding information: This work was jointly supported by the National Natural Science Foundation of China (NSFC) (62175097, 62065013, 11864025, 61865013); Nanchang Hangkong University graduate student innovation special fund project (YC2020-071); Natural Science Foundation of Jiangxi Province (20192ACB20031, 20192ACBL21051 and 20202ACBL202002), Key R&D Projects of the Ministry of Science and Technology of China (2018YFE0115700); Royal Society International Exchanges 2020 Cost Share (NSFC) (IEC\NSFC\201015) and National Natural Science Foundation of China-Royal Society Exchange Programme (62111530153).
Uncontrolled Keywords: Optical microfiber, Biosensor, side-polished optical fiber, Refractive index, Human chorionic gonadotropin (hCG)
Subjects: H600 Electronic and Electrical Engineering
Department: Faculties > Engineering and Environment > Mathematics, Physics and Electrical Engineering
Depositing User: John Coen
Date Deposited: 25 Mar 2022 14:07
Last Modified: 27 May 2022 10:45
URI: http://nrl.northumbria.ac.uk/id/eprint/48758

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