Anti-cancer therapy is associated with long-term epigenomic changes in childhood cancer survivors

Robinson, Natassia, Casement, John, Gunter, Marc J., Huybrechts, Inge, Agudo, Antonio, Barranco, Miguel Rodríguez, Eichelmann, Fabian, Johnson, Theron, Kaaks, Rudolf, Pala, Valeria, Panico, Salvatore, Sandanger, Torkjel M., Schultze, Matthias B., Travis, Ruth C., Tumino, Rosario, Vineis, Paolo, Weiderpass, Elisabete, Skinner, Roderick, Sharp, Linda, Mckay, Jill and Strathdee, Gordon (2022) Anti-cancer therapy is associated with long-term epigenomic changes in childhood cancer survivors. British Journal of Cancer. ISSN 0007-0920 (In Press)

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Official URL: https://doi.org/10.1038/s41416-022-01792-9

Abstract

Childhood cancer survivors (CCS) exhibit significantly increased chronic diseases and premature death. Abnormalities in DNA methylation are associated with development of chronic diseases and reduced life expectancy. We investigated the hypothesis that anti-cancer treatments are associated with long-term DNA methylation changes that could be key drivers of adverse late health effects. Genome-wide DNA methylation was assessed using MethylationEPIC arrays in paired samples (before/after therapy) from 32 childhood cancer patients. Separately, methylation was determined in 32 samples from different adult CCS (mean 22-years post-diagnosis) and compared with cancer-free controls (n = 284). Widespread DNA methylation changes were identified post-treatment in childhood cancer patients, including 146 differentially methylated regions (DMRs), which were consistently altered in the 32 post-treatment samples. Analysis of adult CCS identified matching methylation changes at 107/146 of the DMRs, suggesting potential long-term retention of post-therapy changes. Adult survivors also exhibited epigenetic age acceleration, independent of DMR methylation. Furthermore, altered methylation at the DUSP6 DMR was significantly associated with early mortality, suggesting altered methylation may be prognostic for some late adverse health effects in CCS. These novel methylation changes could serve as biomarkers for assessing normal cell toxicity in ongoing treatments and predicting long-term health outcomes in CCS. [Abstract copyright: © 2022. The Author(s).]

Item Type: Article
Additional Information: Funding information: This work was funded by project grants from The Newcastle Hospitals NHS Charity and the JGW Patterson Foundation. We wish to acknowledge the NECCR for funding of cancer research in the Newcastle University Centre for Cancer.
Subjects: A300 Clinical Medicine
B100 Anatomy, Physiology and Pathology
B900 Others in Subjects allied to Medicine
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Rachel Branson
Date Deposited: 19 Apr 2022 13:17
Last Modified: 19 Apr 2022 13:30
URI: http://nrl.northumbria.ac.uk/id/eprint/48914

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