Highly Sensitive Love Mode Acoustic Wave Platform with SiO2 Wave-Guiding Layer and Gold Nanoparticles for Detection of Carcinoembryonic Antigens

Li, Chong, Zhang, Jikai, Xie, Haiyu, Luo, Jingting, Fu, Chen, Tao, Ran, Li, Honglang and Fu, Yong Qing (2022) Highly Sensitive Love Mode Acoustic Wave Platform with SiO2 Wave-Guiding Layer and Gold Nanoparticles for Detection of Carcinoembryonic Antigens. Biosensors, 12 (7). p. 536. ISSN 2079-6374

[img]
Preview
Text
biosensors-12-00536.pdf - Published Version
Available under License Creative Commons Attribution 4.0.

Download (2MB) | Preview
[img]
Preview
Text
Biosensors accepted manuscript.v7-0717 - clear.pdf - Accepted Version
Available under License Creative Commons Attribution 4.0.

Download (896kB) | Preview
Official URL: https://doi.org/10.3390/bios12070536

Abstract

A highly sensitive and precise Love wave mode surface acoustic wave (SAW) immunosensor based on an ST-cut 90°X quartz substrate and an SiO2 wave-guiding layer was developed to detect cancer-related biomarkers of carcinoembryonic antigens (CEAs). A delay line structure of the SAW device with a resonant frequency of 196 MHz was designed/fabricated, and its surface was functionalized through CEA antibody immobilization. The CEA antibodies were bound with gold nanoparticles and CEA antibodies to form a sandwich structure, which significantly amplified the mass loading effect and enhanced the maximum responses by 30 times. The center frequency of the Love wave immunosensor showed a linear response as a function of the CEA concentration in the range of 0.2–5 ng/mL. It showed a limit of detection of 0.2 ng/mL, and its coefficient of determination was 0.983. The sensor also showed minimal interference from nonspecific adsorptions, thus demonstrating its promise for point-of-care applications for cancer biomarkers.

Item Type: Article
Additional Information: Funding information: This work was financially supported by the Research Project in Fundamental and Application Fields of Guangdong Province (Grant no. 2020A1515110561, 2019A1515111199), Research and Development Program of Guangdong Province (Grant no. 2020B0101040002), Key Research Program of Education Department for Guangdong Province (Grant no. 2020ZDZX2007), National Natural Science Foundation of China (NSFC Grant no. 12104320), Shenzhen Science & Technology Project (Grant no. RCBS20200714114918249, GJHZ20200731095803010), Engineering and Physical Sciences Research Council of UK (EPSRC EP/P018998/1), Special Interest Group for Acoustofluidics from UK Fluids Network (EP/N032861/1), International Exchange Grant (IEC/NSFC/201078) through the Royal Society and NSFC, and EPSRC NetworkPlus in Digitalised Surface Manufacturing (EP/S036180/1).
Uncontrolled Keywords: love wave; biosensors; CEA; AuNPs
Subjects: H800 Chemical, Process and Energy Engineering
Department: Faculties > Engineering and Environment > Mathematics, Physics and Electrical Engineering
Depositing User: Elena Carlaw
Date Deposited: 18 Jul 2022 14:23
Last Modified: 19 Jul 2022 08:30
URI: http://nrl.northumbria.ac.uk/id/eprint/49568

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics