Andreasson, Anders, Karamanou, Danai, Perry, John, Perry, Audrey, Ӧzalp, Faruk, Butt, Tanveer, Morley, Katie, Walden, Hannah, Clark, Stephen, Prabhu, Mahesh, Corris, Paul, Gould, Kate, Fisher, Andrew and Dark, John (2014) The effect of ex vivo lung perfusion on microbial load in human donor lungs. The Journal of Heart and Lung Transplantation, 33 (9). pp. 910-916. ISSN 1053-2498
Full text not available from this repository. (Request a copy)Abstract
Background
Ex vivo lung perfusion (EVLP) has emerged as a technique to potentially recondition unusable donor lungs for transplantation. Beneficial effects of EVLP on physiologic function have been reported, but little is known about the effect of normothermic perfusion on the infectious burden of the donor lung. In this study, we investigated the effect of EVLP on the microbial load of human donor lungs.
Methods
Lungs from 18 human donors considered unusable for transplantation underwent EVLP with a perfusate containing high-dose, empirical, broad-spectrum anti-microbial agents. Quantitative cultures of bacteria and fungi were performed on bronchoalveolar lavage fluid from the donor lung before and after 3 to 6 hours of perfusion. The identification of any organisms and changes in number of colony forming units before and after EVLP were assessed and anti-microbial susceptibilities identified.
Results
Thirteen out of 18 lungs had positive cultures, with bacterial loads significantly decreasing after EVLP. Yeast loads increased when no anti-fungal treatment was given, but were reduced when prophylactic anti-fungal treatment was added to the circuit. Six lungs were ultimately transplanted into patients, all of whom survived to hospital discharge. There was 1 death at 11 months.
Conclusions
Our study shows that EVLP with high-dose, empirical anti-microbial agents in the perfusate is associated with an effective reduction in the microbial burden of the donor lung, a benefit that has not previously been demonstrated.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | lung transplantation, ex vivo lung perfusion, antibiotics, fungicides, donor lung, microbiology |
| Subjects: | B100 Anatomy, Physiology and Pathology C500 Microbiology |
| Department: | Faculties > Health and Life Sciences > Applied Sciences |
| Depositing User: | Paul Burns |
| Date Deposited: | 28 Aug 2014 10:27 |
| Last Modified: | 12 Oct 2019 18:29 |
| URI: | http://nrl.northumbria.ac.uk/id/eprint/17500 |
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