Characterization of a novel pectate lyase, Pel10A, from Pseudomonas cellulosa

Charnock, Simon, Brown, Ian E., Turkenburg, Johan, Black, Gary and Davies, Gideon (2001) Characterization of a novel pectate lyase, Pel10A, from Pseudomonas cellulosa. Acta Crystallographica Section D: Biological Crystallography, 57 (8). pp. 1141-1143. ISSN 0907 4449

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Abstract

Biological recycling of plant material is essential for biosphere maintenance. This perpetual task involves a complex array of enzymes, including extracellular polysaccharide hydrolases and lyases. Whilst much is known about the structure and function of the hydrolases, relatively little is known about the structures and mechanisms of the corresponding lyases. To this end, crystals of the catalytic module of a novel family 10 pectate lyase, Pel10A from Pseudomonas cellulosa, were obtained using polyethylene glycol 2000 monomethylether as a precipitant. They belong to space group P21, with unit-cell parameters a = 47.7, b = 106.1, c = 55.4 Å, β = 92.0°, and have two molecules in the asymmetric unit. The crystals diffract beyond 1.5 Å using synchrotron radiation.

Item Type:	Article
Uncontrolled Keywords:	Pectate lyase
Subjects:	C500 Microbiology
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	Becky Skoyles
Date Deposited:	13 Feb 2015 11:28
Last Modified:	12 Oct 2019 17:29
URI:	http://nrl.northumbria.ac.uk/id/eprint/19276

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