GABAA receptor α1 and α6 subunits mediate cell surface anchoring in cultured cells

Perán, Macarena, Hooper, Helen, Rayner, Sharon, Stephenson, F. Anne and Salas, Ramiro (2004) GABAA receptor α1 and α6 subunits mediate cell surface anchoring in cultured cells. Neuroscience Letters, 364 (2). pp. 67-70. ISSN 0304-3940

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Abstract

The clustering and immobility of γ-aminobutyric acid type A receptors (GABAARs) at discrete and functionally significant domains on the nerve cell surface is an important determinant in the integration of synaptic inputs. To investigate the role that different GABAAR α subunit isoforms play in determining receptor mobility, αxβ3γ2s subunits (where x=α subunit isoforms 1-6) were co-transfected into COS 7 and human embryonic kidney (HEK) 293 cells and the surface mobility of these recombinant complexes was measured by fluorescence photobleach recovery (FPR). In addition, the lateral mobility of endogenous GABAARs in cerebellar granule (CG) cells was measured. We show that the α1 and α6 subunits immobilize recombinant GABAAR in transfected cells. This is consistent with the immobility of native receptors in CG cells, which express α1 and α6.

Item Type:	Article
Uncontrolled Keywords:	fluorescent photobleach recovery, FPR, GABAA receptors, lateral mobility, subunit composition
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	Becky Skoyles
Date Deposited:	02 Feb 2015 15:21
Last Modified:	12 Oct 2019 17:30
URI:	http://nrl.northumbria.ac.uk/id/eprint/19884

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