Characterisation of the Cdc2-related kinase 2 gene from Plasmodium knowlesi and P. berghei

Vinkenoog, Rinke, Sperança, Márcia, Ramesar, Jai, Thomas, Alan, del Portillo, Hernando, Janse, Chris and Waters, Andrew (1998) Characterisation of the Cdc2-related kinase 2 gene from Plasmodium knowlesi and P. berghei. Molecular and Biochemical Parasitology, 95 (2). pp. 229-240. ISSN 0166 6851

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Abstract

The complete sequence of the cdc2-related kinase 2 (CRK2) gene from Plasmodium knowlesi and from P. berghei was determined. In both species, the CRK2 gene is closely linked to an elongation factor 1 α gene. The two CRK2 proteins are highly homologous to the P. falciparum PfPK5 protein. The CRK2 gene of both species is expressed at a low level during the asexual cell-cycle within the host erythrocytes. The P. bergheiCRK2 mRNA is also present in gametocytes and in stages during development in the mosquito, suggesting a role of this protein in different parts of the life cycle. A conserved sequence located in the 5′ untranslated region immediately upstream of the initiator ATG has the potential to form a stem-loop structure. Although the CRK2 protein possesses most of the domains that are conserved among cdc2-proteins, neither a recombinant P. knowlesi CRK2 protein nor a recombinant P. berghei protein was able to complement a yeast cdc28ts mutant. Furthermore and in contrast to the P. falciparum PfPK5 protein, a recombinant monomeric P. knowlesi CRK2 protein showed no kinase activity.

Item Type:	Article
Uncontrolled Keywords:	Malaria; Cdc2; Cell cycle; Kinase
Subjects:	C100 Biology C200 Botany
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	Becky Skoyles
Date Deposited:	09 Apr 2015 14:43
Last Modified:	12 Oct 2019 17:29
URI:	http://nrl.northumbria.ac.uk/id/eprint/22013

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