Molecular detection and isolation from Antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds

Azra Moosvi, S., McDonald, Ian, Pearce, David, Kelly, Donovan and Wood, Ann (2005) Molecular detection and isolation from Antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds. Systematic and Applied Microbiology, 28 (6). pp. 541-554. ISSN 0723-2020

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Abstract

This study is the first demonstration that a diverse facultatively methylotrophic microbiota exists in some Antarctic locations. PCR amplification of genes diagnostic for methylotrophs was carried out with bacterial DNA isolated from 14 soil and sediment samples from ten locations on Signy Island, South Orkney Islands, Antarctica. Genes encoding the mxaF of methanol dehydrogenase, the fdxA for Afipia ferredoxin, the msmA of methanesulfonate monooxygenase, and the 16S rRNA gene of Methylobacterium were detected in all samples tested. The mxaF gene sequences corresponded to those of Hyphomicrobium, Methylobacterium, and Methylomonas. Over 30 pure cultures of methylotrophs were isolated on methanesulfonate, dimethylsulfone, or dimethylsulfide from ten Signy Island lakes. Some were identified from 16S rRNA gene sequences (and morphology) as Hyphomicrobium species, strains of Afipia felis, and a methylotrophic Flavobacterium strain. Antarctic environments thus contain diverse methylotrophic bacteria, growing on various C1-substrates, including C1-sulfur compounds.

Item Type:	Article
Uncontrolled Keywords:	Antarctica; Signy island; Hyphomicrobium; Afipia; Methylobacterium; Methylotrophy; Methanesulfonate; Methanol dehydrogenase
Subjects:	C500 Microbiology
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	Becky Skoyles
Date Deposited:	21 Dec 2015 12:34
Last Modified:	12 Oct 2019 17:30
URI:	http://nrl.northumbria.ac.uk/id/eprint/25137

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