Investigating lipoprotein biogenesis and function in the model Gram-positive bacterium Streptomyces coelicolor

Thompson, Benjamin, Widdick, David, Hicks, Matthew, Chandra, Govind, Sutcliffe, Iain, Palmer, Tracy and Hutchings, Matthew (2010) Investigating lipoprotein biogenesis and function in the model Gram-positive bacterium Streptomyces coelicolor. Molecular Microbiology, 77 (4). pp. 943-957. ISSN 1365-2958

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Abstract

Sec general secretory pathway and then lipidated on a conserved cysteine by the enzyme lipoprotein diacylglycerol transferase (Lgt). The signal peptide is cleaved by lipoprotein signal peptidase (Lsp) to leave the lipid-modified cysteine at the N-terminus of the mature lipoprotein. In all Gram-positive bacteria tested to date this pathway is non-essential and the lipid attaches the protein to the outer leaflet of the cytoplasmic membrane. Here we identify lipoproteins in the model Gram-positive bacterium Streptomyces coelicolor using bioinformatics coupled with proteomic and downstream analysis. We report that Streptomyces species translocate large numbers of lipoproteins out via the Tat (twin arginine translocase) pathway and we present evidence that lipoprotein biogenesis might be an essential pathway in S. coelicolor. This is the first analysis of lipoproteins and lipoprotein biogenesis in Streptomyces and provides the first evidence that lipoprotein biogenesis could be essential in a Gram-positive bacterium. This report also provides the first experimental evidence that Tat plays a major role in the translocation of lipoproteins in a specific bacterium.

Item Type:	Article
Uncontrolled Keywords:	lipoproteins, gram-positive bacteria
Subjects:	C500 Microbiology
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	EPrint Services
Date Deposited:	03 Feb 2011 10:49
Last Modified:	12 Oct 2019 18:26
URI:	http://nrl.northumbria.ac.uk/id/eprint/2753

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