Tissue Microarray Analysis Applied to Bone Diagenesis

Barrios Mello, Rafael, Regis Silva, Maria Regina, Seixas Alves, Maria Teresa, Evison, Martin, Guimarães, Marco Aurélio, Francisco, Rafaella Arrabaça, Dias Astolphi, Rafael and Miazato Iwamura, Edna Sadayo (2017) Tissue Microarray Analysis Applied to Bone Diagenesis. Scientific Reports, 7. p. 39987. ISSN 2045-2322

Text (Full text)
srep39987.pdf - Published Version
Available under License Creative Commons Attribution 4.0.

Download (1MB) | Preview
[img] Other (Full text)
SREP-16-18000B MS.PDF - Accepted Version
Restricted to Repository staff only

Download (1MB)
Official URL: http://dx.doi.org/10.1038/srep39987


Taphonomic processes affecting bone post mortem are important in forensic, archaeological and palaeontological investigations. In this study, the application of tissue microarray (TMA) analysis to a sample of femoral bone specimens from 20 exhumed individuals of known period of burial and age at death is described. TMA allows multiplexing of subsamples, permitting standardized comparative analysis of adjacent sections in 3-D and of representative cross-sections of a large number of specimens. Standard hematoxylin and eosin, periodic acid-Schiff and silver methenamine, and picrosirius red staining, and CD31 and CD34 immunohistochemistry were applied to TMA sections. Osteocyte and osteocyte lacuna counts, percent bone matrix loss, and fungal spheroid element counts could be measured and collagen fibre bundles observed in all specimens. Decalcification with 7 % nitric acid proceeded more rapidly than with 0.5 M EDTA and may offer better preservation of histological and cellular structure. No endothelial cells could be detected using CD31 and CD34 immunohistochemistry. Correlation between osteocytes per lacuna and age at death may reflect reported age-related responses to microdamage. Methodological limitations and caveats, and results of the TMA analysis of post mortem diagenesis in bone are discussed, and implications for DNA survival and recovery considered.

Item Type: Article
Uncontrolled Keywords: Assay systems, Cell death, High-throughput screening, Immunochemistry
Subjects: B800 Medical Technology
F400 Forensic and Archaeological Science
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Paul Burns
Date Deposited: 05 Dec 2016 12:33
Last Modified: 01 Aug 2021 12:36
URI: http://nrl.northumbria.ac.uk/id/eprint/28802

Actions (login required)

View Item View Item


Downloads per month over past year

View more statistics