Identification of the lipooligosaccharide biosynthetic gene cluster from Mycobacterium marinum

Ren, Huipeng, Dover, Lynn, Alexander, David, Islam, Salim, Chen, Jeffrey, Besra, Gurdyal and Liu, Jun (2007) Identification of the lipooligosaccharide biosynthetic gene cluster from Mycobacterium marinum. Molecular Microbiology, 63 (5). pp. 1345-1359. ISSN 0950-382X

Full text not available from this repository. (Request a copy)
Official URL:


Lipooligosaccharides (LOSs) are antigenic glycolipids that are present in some species of Mycobacterium including the Canetti strain of M. tuberculosis. The core LOS structures from several mycobacterial organisms have been established, but the biosynthetic pathways of LOSs remain unknown. In this study, we describe two transposon insertion mutants of M. marinum that exhibit altered colony morphology. Cell wall analysis reveals that the MRS1271 mutant is defective in the synthesis of LOS-II, whereas the MRS1178 mutant accumulates an intermediate between LOS-I and -II. The genetic lesions were localized to two genes, MM2309 and MM2332. MM2309 encodes a UDP-glucose dehydrogenase that is involved in the synthesis of d-xylose. MM2332 is predicted to encode a decarboxylase. These two genes and a previously identified losA gene are localized in a gene cluster likely to be involved in the biosynthesis of LOSs. Our results also show that LOSs play an important role in sliding motility, biofilm formation, and infection of host macrophages. Taken together, our studies have identified, for the first time, a LOS biosynthetic locus. This is an important step in assessing the differential distribution of LOSs among Mycobacterium species and understanding the role of LOSs in mycobacterial virulence.

Item Type: Article
Subjects: C500 Microbiology
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: EPrint Services
Date Deposited: 18 Dec 2008 14:42
Last Modified: 31 Jul 2021 08:38

Actions (login required)

View Item View Item


Downloads per month over past year

View more statistics