Identification of B2 Bradykinin Binding Sites on Cultured Cortical Astrocytes

Cholewinski, A. J., Stevens, G., McDermott, Alison and Wilkin, G. P. (1991) Identification of B2 Bradykinin Binding Sites on Cultured Cortical Astrocytes. Journal of Neurochemistry, 57 (4). pp. 1456-1458. ISSN 0022-3042

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Abstract

Bradykinin was found to bind to specific high-affinity sites in cultured cortical astrocytes from rat brain, and this binding appeared to be specific for the B2 bradykinin receptor subtype. Nonlinear regression analysis of saturation experiments using a computer programme revealed a single KD of 16.6 ± 2.6 nM and a Bmax of 352.2 ±30.7 fmol/mg of protein. These results indicate that astrocytes possess bradykinin receptors and that these are predominantly of the B2 subtype.

Item Type:	Article
Uncontrolled Keywords:	Bradykinin; Bradykinin receptors; Astrocytes; Cell culture
Subjects:	C800 Psychology C900 Others in Biological Sciences
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	Becky Skoyles
Date Deposited:	25 May 2017 08:14
Last Modified:	12 Oct 2019 17:29
URI:	http://nrl.northumbria.ac.uk/id/eprint/30850

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