Houweling, Diane, Brook, Gary, Gieling, Roben, Veldman, H., Woodhams, P., Nacimiento, Wilhelm, Noth, Johannes, Bär, Dop and Joosten, Elbert (1999) Differential distribution of immunoreactivity in the developing rat spinal cord revealed by the monoclonal antibody Py. Developmental Brain Research, 116 (1). pp. 87-96. ISSN 0165-3806
Full text not available from this repository.Abstract
Monoclonal antibody Py was developed as a useful tool for the identification of large diameter neurons of the adult rat central nervous system [Woodhams et al., J. Neurosci., 9 (1989) 2170–2181]. Here, we present a detailed light-microscopic study of the distribution of Py-immunoreactivity in the developing rat spinal cord. The first cells which demonstrated Py-immunoreactivity were the motoneurons in layer IX of the gray matter at embryonic day 15. These cells, including their axons and dendrites, remained Py-immunoreactive throughout subsequent developmental stages into adulthood and were the most intensely stained cells in the adult rat spinal cord. Other cell populations which became Py-immunoreactive during development were neurons in layers III–VIII, and large-to-medium diameter neurons of the dorsal root ganglion (DRG). Transient Py-immunoreactivity was observed in the distal portions of DRG axons as well as in the ascending fibers in the dorsal funiculus. Py-immunoreactive fibers could be detected in the ventral most part of the dorsal funiculus (corticospinal tract area), even at embryonic ages prior to the arrival of corticospinal fibers. The localization and transient expression of the antigen recognized by the Py-antibody in developing rat spinal cord strongly suggests an important role of this molecule in stabilization and/or plasticity of the neuronal cytoskeleton. The results presented here form the foundation for the use of Py-immunocytochemistry to study well-defined cell populations under a range of experimental and pathological conditions.
Item Type: | Article |
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Uncontrolled Keywords: | Cytoskeleton, Motoneuron, MAP, DRG, Spinal cord, Development |
Subjects: | B900 Others in Subjects allied to Medicine C900 Others in Biological Sciences |
Department: | Faculties > Health and Life Sciences > Applied Sciences |
Depositing User: | Becky Skoyles |
Date Deposited: | 17 Jul 2018 10:40 |
Last Modified: | 11 Oct 2019 19:45 |
URI: | http://nrl.northumbria.ac.uk/id/eprint/35022 |
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