ABC medium, a new chromogenic agar for the selective isolation of Salmonella spp.

Perry, John, Ford, Michael, Taylor, Jeffrey, Jones, Amanda, Freeman, Roger and Gould, Frances Kate (1999) ABC medium, a new chromogenic agar for the selective isolation of Salmonella spp. Journal of Clinical Microbiology, 37 (3). pp. 766-768. ISSN 0095-1137

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Abstract

We describe a new chromogenic agar medium, ABC medium (αβ-chromogenic medium), which includes two substrates, 3,4-cyclohexenoesculetin-β-D-galactoside and 5-bromo-4-chloro-3-indolyl-α-D-galactopyranoside, to facilitate the selective isolation of Salmonella spp. This medium exploits the fact that Salmonella spp. may be distinguished from other members of the familyEnterobacteriaceae by the presence of α-galactosidase activity in the absence of β-galactosidase activity. A total of 1,022 strains of Salmonella spp. and 300 other gram-negative strains were inoculated onto this medium. Of these, 1,019 (99.7%) strains of Salmonella spp. produced a characteristic green colony, whereas only 1 strain (0.33%) of non-Salmonellaproduced a green colony. A total of 283 stool samples were cultured onto desoxycholate citrate (DC) agar and ABC medium by direct inoculation and after selective enrichment in selenite broth. Overall, the sensitivity and specificity were superior for ABC medium (100 and 90.5%, respectively) than for DC agar (88 and 26.9%, respectively). We conclude that ABC medium offers a high degree of specificity for the detection of Salmonella spp. in stool samples.

Item Type:	Article
Uncontrolled Keywords:	enterobacteriaceae, salmonella
Subjects:	C500 Microbiology
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	EPrint Services
Date Deposited:	22 May 2009 10:44
Last Modified:	12 Oct 2019 17:29
URI:	http://nrl.northumbria.ac.uk/id/eprint/3557

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