The major histocompatibility complex class I immunopeptidome of extracellular vesicles

Synowsky, Silvia, Shirran, Sally, Cooke, Fiona, Antoniou, Antony, Botting, Catherine and Powis, Simon (2017) The major histocompatibility complex class I immunopeptidome of extracellular vesicles. The Journal of Biological Chemistry, 292 (41). pp. 17084-17092. ISSN 0021-9258

Full text not available from this repository.

Abstract

Extracellular vesicles (EVs) are released by most cell types and have been associated with multiple immunomodulatory functions. MHC class I molecules have crucial roles in antigen presentation and in eliciting immune responses and are known to be incorporated into EVs. However, the MHC class I immunopeptidome of EVs has not been established. Here, using a small-scale immunoisolation of the antigen serotypes HLA-A*02:01 and HLA-B*27:05 expressed on the Epstein-Barr virus-transformed B cell line Jesthom and MS of the eluted peptides from both cells and EVs, we identified 516 peptides that bind either HLA-A*02:01 or HLA-B*27:05. Of importance, the predicted serotype-binding affinities and peptide-anchor motifs did not significantly differ between the peptide pools isolated from cells or EVs, indicating that during EV biogenesis, no obvious editing of the MHC class I immunopeptidome occurs. These results, for the first time, establish EVs as a source of MHC class I peptides that can be used for the study of the immunopeptidome and in the discovery of potential neoantigens for immunotherapies.

Item Type:	Article
Subjects:	C700 Molecular Biology, Biophysics and Biochemistry
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	Becky Skoyles
Date Deposited:	07 Nov 2018 15:25
Last Modified:	11 Oct 2019 18:45
URI:	http://nrl.northumbria.ac.uk/id/eprint/36543

Downloads