Atomic force microscope based biomolecular force-clamp measurements using a micromachined electrostatic actuator

Torun, Hamdi, Finkler, Ofer and Degertekin, F. Levent (2012) Atomic force microscope based biomolecular force-clamp measurements using a micromachined electrostatic actuator. Ultramicroscopy, 122. pp. 26-31. ISSN 0304-3991

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Abstract

The authors describe a method for biomolecular force clamp measurements using atomic force microscope (AFM) cantilevers and micromachined membrane-based electrostatic actuators. The actuators comprise of Parylene membranes with embedded side actuation electrodes and are fabricated on a silicon substrate. The devices have a displacement range of 1.8 μm with 200 V actuation voltage, and displacement uncertainty is 0.8 nm, including the noise and drift. The settling time, limited by the particular amplifier is 5 ms, with an inherent range down to 20 μs. A force clamp measurement setup using these actuators in a feedback loop has been used to measure bond life-times between human IgG and anti-human IgG molecules to demonstrate the feasibility of this method for biological experiments. The experimental findings are compared with a molecular pulling experiment and the results are found to be in good agreement.

Item Type:	Article
Uncontrolled Keywords:	Atomic force microscopy, Microsystems, Force spectroscopy, Single molecule mechanics
Subjects:	F300 Physics
Department:	Faculties > Engineering and Environment > Mathematics, Physics and Electrical Engineering
Depositing User:	Becky Skoyles
Date Deposited:	14 Dec 2018 09:53
Last Modified:	11 Oct 2019 15:02
URI:	http://nrl.northumbria.ac.uk/id/eprint/37264

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