Histone deacetylase inhibition redistributes topoisomerase IIb from heterochromatin to euchromatin

Cowell, Ian, Papageorgiou, Nikolaos, Padget, Kay, Watters, Gary and Austin, Caroline (2011) Histone deacetylase inhibition redistributes topoisomerase IIb from heterochromatin to euchromatin. Nucleus, 2 (1). pp. 61-71. ISSN 1949-1034

Full text not available from this repository. (Request a copy)

Abstract

The genome is organised into large scale structures in the interphase nucleus. Pericentromeric heterochromatin represents one such compartment characterised by histones H3 and H4 tri-methylated at K9 and K20 respectively and with a correspondingly low level of histone acetylation. HP1 proteins are concentrated in pericentric heterochromatin and histone deacetylase inhibitors such as trichostatin A (TSA) promote hyperacetylation of heterochromatic nucleosomes and the dispersal of HP1 proteins. We observed that in mouse cells, which contain prominent heterochromatin, DNA topoisomerase IIb (topoIIb) is also concentrated in heterochromatic regions. Similarly, a detergent-resistant fraction of topoIIb is associated with heterochromatin in human cell lines. Treatment with TSA displaced topoIIb from the heterochromatin with similar kinetics to the displacement of HP1b. Topoisomerase II is the cellular target for a number of clinically important cytotoxic anti-cancer agents known collectively as topoisomerase poisons, and it has been previously reported that histone deacetylase inhibitors can sensitise cells to these drugs. While topoIIa appears to be the major target for most topoisomerase poisons, histone deacetylase-mediated potentiation of these drugs is dependent on topoIIb. We find that while prior treatment with TSA did not increase the quantity of etoposide-mediated topoIIb-DNA covalent complexes, it did result in a shift in their distribution from a largely heterochromatin-associated to a pan-nuclear pattern. We suggest that this redistribution of topoIIb converts this isoform of topoII to a effective relevant target for topoisomerase poisons.

Item Type:	Article
Uncontrolled Keywords:	histone acetyl transferase, chromatin remodelling, etoposide, nucleolus, DNA damage
Subjects:	C900 Others in Biological Sciences
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	Ay Okpokam
Date Deposited:	12 Dec 2011 22:32
Last Modified:	12 Oct 2019 18:26
URI:	http://nrl.northumbria.ac.uk/id/eprint/4040

Downloads