Feng, Luying and Torun, Hamdi (2020) Miniaturized Magnetic Bead-Actuators for Force-Clamp Spectroscopy-Based Single-Molecule Measurements. Ultramicroscopy, 209. p. 112888. ISSN 0304-3991
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Abstract
Force-clamp spectroscopy can mimic the physiological conditions for the proteins under investigation. In addition, it is a direct way of observing the relationship between bond lifetime and molecular forces. However, traditional force-clamp methods rely on active feedback controllers that can introduce artefacts. In this work, we introduce a new method to enable force-clamp spectroscopy without a need for an active feedback. The method is based on miniaturized magnetic beads offering improved stability. As a case study, we performed force-clamp experiments using biotin-streptavidin molecule pairs with and without active feedback. Our results demonstrate the feasibility of force-clamp experiments without feedback and illustrate the advantages of our method.
| Item Type: | Article |
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| Uncontrolled Keywords: | Atomic force microscopy, Single-Molecule Mechanics, Force-clamp Spectroscopy, Biotin, Streptavidin |
| Subjects: | F300 Physics H600 Electronic and Electrical Engineering H800 Chemical, Process and Energy Engineering |
| Department: | Faculties > Engineering and Environment > Mathematics, Physics and Electrical Engineering |
| Depositing User: | Elena Carlaw |
| Date Deposited: | 11 Nov 2019 13:48 |
| Last Modified: | 31 Jul 2021 13:31 |
| URI: | http://nrl.northumbria.ac.uk/id/eprint/41407 |
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