Static and Dynamic Systems in Rickettsia slovaca Life Cycle Evaluated by Quantitative Real-Time Polymerase Chain Reaction

Špitalská, Eva, Sparagano, Olivier and Boldiš, Vojtech (2010) Static and Dynamic Systems in Rickettsia slovaca Life Cycle Evaluated by Quantitative Real-Time Polymerase Chain Reaction. Transboundary and Emerging Diseases, 57 (1-2). pp. 70-71. ISSN 1865-1674

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Abstract

Quantitative real-time polymerase chain reaction was used to characterize the growth of Rickettsia slovaca, a tick-borne pathogen transmitted by Dermacentor reticulatus and D. marginatus ticks, in static (L929 and Vero cells) and dynamic (D. marginatus and Ixodes ricinus ticks) cultivation systems. The highest points of bacterial multiplication and the time-spans between the inoculum and the maximum of rickettsial copies were increased in consecutive order from eukaryotic cells, I. ricinus to D. marginatus systems. In dynamic system, multiplication maximum of R. slovaca was achieved 9 days earlier in I. ricinus; however, the number of rickettsial DNA copies was ∼3.6 × 106 more in D. marginatus.

Item Type:	Article
Uncontrolled Keywords:	Dermacentor marginatus, ixodes ricinus, L929, vero, rickettsia slovaca, quantitative real-time polymerase chain reaction
Subjects:	C100 Biology C500 Microbiology C700 Molecular Biology, Biophysics and Biochemistry
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	Ellen Cole
Date Deposited:	11 Jan 2012 13:01
Last Modified:	12 Oct 2019 18:26
URI:	http://nrl.northumbria.ac.uk/id/eprint/4501

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