Immunological Control of the Poultry Red Mite: the use of whole mite antigens as a vaccine candidate

Arkle, Sam, Harrington, David, Kaiser, Pete, Rothwell, Lisa, de Luna, Carlos, George, David, Guy, Jonathan and Sparagano, Olivier (2008) Immunological Control of the Poultry Red Mite: the use of whole mite antigens as a vaccine candidate. Annals of the New York Academy of Sciences, 1149 (1). pp. 36-40. ISSN 0077-8923

Full text not available from this repository.
Official URL:


In the current study whole poultry red mite antigens were extracted and birds were immunized subcutaneously with either antigen in adjuvant (antigen group) or PBS in adjuvant (control group). Immune responses of birds following immunization were investigated by ELISA and Western blotting, while vaccine efficacy was assessed by feeding of red mites on birds. Immunized birds showed a significant (P < 0.05) increase in IgY titers after immunization compared to controls, while immunoglobulin A (IgA) and IgM did not change significantly. However, the antigen group had a generally higher increase in all immunoglobulin titers compared to the controls. Western blotting identified a number of protein bands at different molecular weights, although these were not different between treatments. PCR analysis of whole mite protein identified bacterial DNA that might have confounded immunological data. In addition, there was a trend toward reduced survival rate of red mites feeding on antigen-immunized birds, but the difference was not statistically significant compared to controls. This study demonstrates the potential for somatic red mite antigens to stimulate an antibody-mediated immune response, although this response did not confer protection to birds.

Item Type: Article
Uncontrolled Keywords: poultry red mite, Dermanyssus gallinae, vaccine, antigen, ectoparasite, chicken
Subjects: C100 Biology
C500 Microbiology
C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Ellen Cole
Date Deposited: 12 Jan 2012 11:53
Last Modified: 26 May 2023 08:22

Actions (login required)

View Item View Item


Downloads per month over past year

View more statistics