Development of Specific Oligonucleotide Probes to DetectVibrioSpecies

Molini, Umberto, Thanantong, Narut, Giangaspero, Annunziata and Sparagano, Olivier (2008) Development of Specific Oligonucleotide Probes to DetectVibrioSpecies. Annals of the New York Academy of Sciences, 1149 (1). pp. 312-314. ISSN 0077-8923

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Abstract

Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V. parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks.

Item Type:	Article
Uncontrolled Keywords:	vibrio, reverse line blot, PCR, diagnostic
Subjects:	C100 Biology C500 Microbiology C700 Molecular Biology, Biophysics and Biochemistry
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	Ellen Cole
Date Deposited:	12 Jan 2012 14:56
Last Modified:	12 Oct 2019 17:31
URI:	http://nrl.northumbria.ac.uk/id/eprint/4536

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