The Stool Volatile Metabolome of Pre-Term Babies

Frau, Alessandra, Lett, Lauren, Slater, Rachael, Young, Greg, Stewart, Christopher J., Berrington, Janet, Hughes, David M., Embleton, Nicholas and Probert, Chris (2021) The Stool Volatile Metabolome of Pre-Term Babies. Molecules, 26 (11). p. 3341. ISSN 1420-3049

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The fecal metabolome in early life has seldom been studied. We investigated its evolution in pre-term babies during their first weeks of life. Multiple (n = 152) stool samples were studied from 51 babies, all <32 weeks gestation. Volatile organic compounds (VOCs) were analyzed by headspace solid phase microextraction gas chromatography mass spectrometry. Data were interpreted using Automated Mass Spectral Deconvolution System (AMDIS) with the National Institute of Standards and Technology (NIST) reference library. Statistical analysis was based on linear mixed modelling, the number of VOCs increased over time; a rise was mainly observed between day 5 and day 10. The shift at day 5 was associated with products of branched-chain fatty acids. Prior to this, the metabolome was dominated by aldehydes and acetic acid. Caesarean delivery showed a modest association with molecules of fungal origin. This study shows how the metabolome changes in early life in pre-term babies. The shift in the metabolome 5 days after delivery coincides with the establishment of enteral feeding and the transition from meconium to feces. Great diversity of metabolites was associated with being fed greater volumes of milk.

Item Type: Article
Additional Information: Funding information: This research was funded by National Institute for Health Research (NIHR) Efficacy and Mechanistic Evaluation programme (EME) grant number 13/122/02.
Uncontrolled Keywords: metabolome; feces; neonates; fermentation; protein; carbohydrate; short chain fatty acid
Subjects: C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Elena Carlaw
Date Deposited: 04 Jun 2021 08:46
Last Modified: 31 Jul 2021 11:06

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