Lasonder, Edwin, Janse, Chris J., van Gemert, Geert-Jan, Mair, Gunnar R., Vermunt, Adriaan M. W., Douradinha, Bruno G., van Noort, Vera, Huynen, Martijn A., Luty, Adrian J. F., Kroeze, Hans, Khan, Shahid M., Sauerwein, Robert W., Waters, Andrew P., Mann, Matthias and Stunnenberg, Hendrik G. (2008) Proteomic Profiling of Plasmodium Sporozoite Maturation Identifies New Proteins Essential for Parasite Development and Infectivity. PLoS Pathogens, 4 (10). e1000195. ISSN 1553-7374
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Abstract
Plasmodium falciparum sporozoites that develop and mature inside an Anopheles mosquito initiate a malaria infection in humans. Here we report the first proteomic comparison of different parasite stages from the mosquito—early and late oocysts containing midgut sporozoites, and the mature, infectious salivary gland sporozoites. Despite the morphological similarity between midgut and salivary gland sporozoites, their proteomes are markedly different, in agreement with their increase in hepatocyte infectivity. The different sporozoite proteomes contain a large number of stage specific proteins whose annotation suggest an involvement in sporozoite maturation, motility, infection of the human host and associated metabolic adjustments. Analyses of proteins identified in the P. falciparum sporozoite proteomes by orthologous gene disruption in the rodent malaria parasite, P. berghei, revealed three previously uncharacterized Plasmodium proteins that appear to be essential for sporozoite development at distinct points of maturation in the mosquito. This study sheds light on the development and maturation of the malaria parasite in an Anopheles mosquito and also identifies proteins that may be essential for sporozoite infectivity to humans.
| Item Type: | Article |
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| Additional Information: | Funding information: Part of this work was supported by the Dutch Science Foundation (NWO/Genomics, grant number 050-10-053) and The Wellcome Trust Functional Genomics Initiative. EL and SMK were supported by a programme from the Dutch NWO/Genomics, AMWV and GJvG by Maltrans (EU Framework Programme V), GRM was supported by BioMalPar and is a recipient of a Netherlands Genomics Initiative HORIZON Project Grant (050-71-061), and SMK is supported by TI-Pharma (T4-102). This study was supported by the WHO. |
| Subjects: | C700 Molecular Biology, Biophysics and Biochemistry |
| Department: | Faculties > Health and Life Sciences > Applied Sciences |
| Depositing User: | Elena Carlaw |
| Date Deposited: | 07 Jun 2021 12:02 |
| Last Modified: | 31 Jul 2021 11:05 |
| URI: | http://nrl.northumbria.ac.uk/id/eprint/46363 |
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