Characterizing Marathon-Induced Metabolic Changes Using 1H-NMR Metabolomics

Bester, Rachelle, Stander, Zinandré, Mason, Shayne, Keane, Karen, Howatson, Glyn, Clifford, Tom, Stevenson, Emma J. and Loots, Du Toit (2021) Characterizing Marathon-Induced Metabolic Changes Using 1H-NMR Metabolomics. Metabolites, 11 (10). p. 656. ISSN 2218-1989

metabolites-11-00656.pdf - Published Version
Available under License Creative Commons Attribution 4.0.

Download (931kB) | Preview
Bester_2021_MANUSCRIPT_Characterizing_marathon_induced_metabolic_changes_using_1H_NMR.pdf - Accepted Version
Available under License Creative Commons Attribution 4.0.

Download (889kB) | Preview
Official URL:


Although physical activity is a health-promoting, popular global pastime, regular engage-ment in strenuous exercises, such as long-distance endurance running races, has been associated with a variety of detrimental physiological and immunological health effects. The resulting altered physiological state has previously been associated with fluctuations in various key metabolite con-centrations; however, limited literature exists pertaining to the global/holistic metabolic changes that are induced by such. This investigation subsequently aims at elucidating the metabolic changes induced by a marathon by employing an untargeted proton nuclear magnetic resonance (1H-NMR) spectrometry metabolomics approach. A principal component analysis (PCA) plot revealed a natu-ral differentiation between pre- and post-marathon metabolic profiles of the 30-athlete cohort, where 17 metabolite fluctuations were deemed to be statistically significant. These included reduced concentrations of various amino acids (AA) along with elevated concentrations of ketone bodies, glycolysis, tricarboxylic acid (TCA) cycle, and AA catabolism intermediates. Moreover, elevated concentrations of creatinine and creatine in the post-marathon group supports previous findings of marathon-induced muscle damage. Collectively, the results of this investigation characterize the strenuous metabolic load induced by a marathon and the consequential regulation of main energy-producing pathways to accommodate this, and a better description of the cause of the physiological changes seen after the completion of a marathon.

Item Type: Article
Additional Information: Funding information: This work is based on research supported in part by the National Research Foundation of South Africa (grant number 130450). Opinions, findings, and conclusions/recommendations expressed in this publication is that of the authors and does not necessarily represent those of the NRF.
Uncontrolled Keywords: endurance races, marathon, metabolites, untargeted metabolomics, 1H-NMR spectrometry, serum metabolome
Subjects: C600 Sports Science
C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Health and Life Sciences > Sport, Exercise and Rehabilitation
Depositing User: Rachel Branson
Date Deposited: 23 Sep 2021 09:54
Last Modified: 15 Oct 2021 14:00

Actions (login required)

View Item View Item


Downloads per month over past year

View more statistics