Ultrasensitive Leaky Surface Acoustic Wave Immunosensor for Real-Time Detection of Alpha-Fetoprotein in Biological Fluids

Rauf, Sana, Qazi, Hafiz Imran Ahmad, Luo, Jingting, Fu, Chen, Tao, Ran, Rauf, Sajid, Yang, Lei, Li, Honglang and Fu, Yong Qing (2021) Ultrasensitive Leaky Surface Acoustic Wave Immunosensor for Real-Time Detection of Alpha-Fetoprotein in Biological Fluids. Chemosensors, 9 (11). p. 311. ISSN 2227-9040

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Official URL: https://doi.org/10.3390/chemosensors9110311


We propose an ultrasensitive leaky surface acoustic wave (LSAW) immunosensor based on molybdenum disulfide @ cuprous oxide—gold (MoS2@Cu2O-Au) nanoparticles and subsequent gold staining for the detection of alpha-fetoprotein (AFP). MoS2@Cu2O-Au nanoparticles, with their large specific surface area and good biocompatibility, not only capture the secondary antibodies (Ab2) but also amplify the mass loading effect of the acoustic wave sensor in the detection of AFP. The immunosensor signals are further amplified upon injection of gold staining solution. The developed immunosensor achieved a low detection limit of 5.5 and 25.0 pg/mL with and without gold staining, respectively. The immunosensor demonstrated its efficiency for the quantitative detection of AFP in complex biological fluids, including human serum and saliva samples, with excellent selectivity and long-term stability, showing great potential for the quantification of AFP in clinical diagnosis.

Item Type: Article
Additional Information: Funding information: This work was financially supported by the Key Research and Development Program of Guangdong Province (Grant No.: 2020B0101040002), Special Projects in Key Fields of Colleges in Guangdong Province (2020ZDZX2097), Research Project in Fundamental and Application Fields of Guangdong Province (2020A1515110561), Guangdong Basic and Applied Basic Research Foundation (2019A1515111199), and Shenzhen Science & Technology Project (Grant No. JCYJ20180507182106754, JCYJ20180507182439574, RCBS20200714114918249, GJHZ20200073109583010), the Engineering and Physical Sciences Research Council of UK (EPSRC EP/P018998/1), International Exchange Grant (IEC/NSFC/201078) through Royal Society and the National Natural Science Foundation of China, and EPSRC NetworkPlus in Digitalised Surface.
Uncontrolled Keywords: leaky surface acoustic wave (LSAW); immunosensor; AFP; MoS2@Cu2O-Au; gold staining
Subjects: F100 Chemistry
H800 Chemical, Process and Energy Engineering
Department: Faculties > Engineering and Environment > Mathematics, Physics and Electrical Engineering
Depositing User: Elena Carlaw
Date Deposited: 04 Nov 2021 10:07
Last Modified: 04 Nov 2021 10:15
URI: http://nrl.northumbria.ac.uk/id/eprint/47627

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