Highly transparent liquid marble in liquid (HT-LMIL) as 3D miniaturized reactor for real-time bio-/chemical assays

Zhao, Zhijian, Yao, Xiaoxue, Zhao, Wen, Shi, Bo, Sridhar, Sreepathy, Pu, Yuan, Pramana, Stevin, Wang, Dan and Wang, Steven (2022) Highly transparent liquid marble in liquid (HT-LMIL) as 3D miniaturized reactor for real-time bio-/chemical assays. Chemical Engineering Journal, 443. p. 136417. ISSN 1385-8947

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Official URL: https://doi.org/10.1016/j.cej.2022.136417


Traditional liquid marbles (LMs), are liquid droplets encapsulated by hydrophobic particles offering special three-dimensional (3D) stereo-architectures with structurally and functionally confined environments. However, the particle armors impede optical characterization in chemical analysis and process monitoring for fluid reactions. For the first time, we manipulated the liquid–solid interface by the paradigm of liquid marbles in liquid (LMIL) strategy, in which LMs are coated by diverse particles immersed in organic mediums rendering an optically transparent micro-reactor system without compromising on its original key features. We demonstrated comparable transmittance of highly transparent liquid marble in liquid (HT-LMIL) towards naked water droplets via UV–vis absorption spectroscopic detection. Besides, its function as an oil immersion lens gives higher resolution in optical spectroscopic characterization. The high optical transparency of LMIL enables real-time and in situ monitoring of yeast cells’ proliferation and viability via layer-by-layer, encouraging them to differentiate in suspension without anchoring. Such multifaceted characteristics of HT-LMIL based platform offer optically transparent and functionally promising micro/bio-reactors with future explorations for high-throughput chemical assays.

Item Type: Article
Additional Information: Funding Information: Z.J. Zhao and X.X. Yao contributed equally to this work. We are grateful for final support from National Key Research and Development Program of China (2021YFC3001602). This research was also financially supported by grants 9610469 and 7005636 from the City University of Hong Kong.
Uncontrolled Keywords: 3D miniaturized reactor, Cell culture, Chemical assays, HT-LMIL, Liquid marble
Subjects: H300 Mechanical Engineering
Department: Faculties > Engineering and Environment > Mechanical and Construction Engineering
Depositing User: Rachel Branson
Date Deposited: 29 Apr 2022 09:49
Last Modified: 19 Apr 2023 08:00
URI: https://nrl.northumbria.ac.uk/id/eprint/48998

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