Effects of norfloxacin, copper, and their interactions on microbial communities in estuarine sediment

Chen, Xiaohan, Chen, Jinjin, Yu, Xiaoxuan, Sanganyado, Edmond, Wang, Lin, Li, Ping and Liu, Wenhua (2022) Effects of norfloxacin, copper, and their interactions on microbial communities in estuarine sediment. Environmental Research, 212 (Part D). p. 113506. ISSN 0013-9351

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Official URL: https://doi.org/10.1016/j.envres.2022.113506


The discharge of antibiotics and metals in estuaries is of great concern since they threaten microbial communities that are critical for maintaining ecosystem function. To understand single and combined effects of norfloxacin (0–20 μg g−1) and copper (40 μg g−1) on microbial ecology in estuaries, we evaluated changes in bacteria population, inhibition rates, and microbial composition in estuarine sediments over a 28-day period. Bacteria population significantly decreased following single and combined exposure to norfloxacin and copper throughout the incubation period, except on Day 28 in treatments exposed to copper, 20 μg g−1 norfloxacin, or both. These three treatment groups had lower Shannon diversity and Simpson's indices on Day 28 than other treatments and the controls suggesting recovery in bacteria population did not correspond with recovery in richness and evenness. Furthermore, functional predictions revealed that the effect of time and contaminants were significantly different on some microbial community functions on Day 28, especially the combination of Cu and high concentration NFX, including aerobic chemoheterotrophy, methanol oxidation and methylotrophy. Thus, norfloxacin and copper had significant adverse effects on microbial communities in estuarine sediments; however, the combined effects were variable and depended on exposure duration and antibiotic concentration.

Item Type: Article
Additional Information: Funding information: The authors gratefully acknowledge the financial support by the National Natural Science Foundation of China (grant numbers 41676166 and 41776174), Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (grant number GML2019ZD0606), and Shantou University Research Start-Up Program (grant number NTF20002), Li Ka Shing Foundation Interdisciplinary Research Project (grant number 2020LKSFG04E).
Uncontrolled Keywords: Antibiotics, Benthic microorganisms, Mixture effects, Microbial ecology, Ecological function prediction
Subjects: C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Elena Carlaw
Date Deposited: 30 May 2022 10:55
Last Modified: 30 May 2022 11:15
URI: http://nrl.northumbria.ac.uk/id/eprint/49214

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