Validation of somatic cell score-associated SNPs from Holstein cattle in Sudanese Butana and Butana × Holstein crossbred cattle

Elzaki, Salma, Korkuc, Paula, Arends, Danny, Reissmann, Monika, Rahmatalla, Siham A. and Brockmann, Gudrun A. (2022) Validation of somatic cell score-associated SNPs from Holstein cattle in Sudanese Butana and Butana × Holstein crossbred cattle. Tropical Animal Health and Production, 54 (1). p. 50. ISSN 0049-4747

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The Bos indicus zebu cattle Butana is the most commonly used indigenous dairy cattle breed in Sudan. In the last years, high-yielding Holstein dairy cattle were introgressed into Butana cattle to improve their milk yield and simultaneously keep their good adaption to extreme environmental conditions. With the focus on the improvement of milk production, other problems arose such as an increased susceptibility to mastitis. Thus, genetic selection for mastitis resistance should be considered to maintain healthy and productive cows. In this study, we tested 10 single nucleotide polymorphisms (SNPs) which had been associated with somatic cell score (SCS) in Holstein cattle for association with SCS in 37 purebred Butana and 203 Butana × Holstein crossbred cattle from Sudan. Animals were genotyped by competitive allele-specific PCR assays and association analysis was performed using a linear mixed model. All 10 SNPs were segregating in the crossbred Butana × Holstein populations, but only 8 SNPs in Sudanese purebred Butana cattle. The SNP on chromosome 13 was suggestively associated with SCS in the Butana × Holstein crossbred population (rs109441194, 13:79,365,467, PBF = 0.054) and the SNP on chromosome 19 was significantly associated with SCS in both populations (rs41257403, 19:50,027,458, Butana: PBF = 0.003, Butana × Holstein: PBF = 6.2 × 10−16). The minor allele of both SNPs showed an increase in SCS. Therefore, selection against the disadvantageous minor allele could be used for genetic improvement of mastitis resistance in the studied populations. However, investigations in a bigger population and across the whole genome are needed to identify additional genomic loci.

Item Type: Article
Additional Information: Funding Information: Open Access funding enabled and organized by Projekt DEAL. SE was supported by the Sudanese Ministry of Higher Education and Scientific research and the Fiat panis foundation. The collection of data and samples was supported by the Ministry of Higher Education and Scientific Research Sudan.
Uncontrolled Keywords: Association analysis, Bos indicus, Genotyping, Mastitis, SNPs
Subjects: C700 Molecular Biology, Biophysics and Biochemistry
D200 Clinical Veterinary Medicine and Dentistry
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: John Coen
Date Deposited: 12 Sep 2022 13:15
Last Modified: 12 Sep 2022 13:30

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