A deletion containing a CTCF-element in intron 8 of the Bbs7 gene is partially responsible for juvenile obesity in the Berlin Fat Mouse

Krause, Florian, Mohebian, Kourosh, Delpero, Manuel, Hesse, Deike, Kühn, Ralf, Arends, Danny and Brockmann, Gudrun A. (2022) A deletion containing a CTCF-element in intron 8 of the Bbs7 gene is partially responsible for juvenile obesity in the Berlin Fat Mouse. Mammalian Genome, 33 (3). pp. 465-470. ISSN 0938-8990

s00335-021-09938-5.pdf - Published Version
Available under License Creative Commons Attribution 4.0.

Download (676kB) | Preview
Official URL: https://doi.org/10.1007/s00335-021-09938-5


The Berlin Fat Mouse Inbred (BFMI) line is a model for juvenile obesity. Previous studies on crosses between BFMI and C57Bl/6N (B6N) have identified a recessive defect causing juvenile obesity on chromosome 3 (jObes1). Bbs7 was identified as the most likely candidate gene for the observed effect. Comparative sequence analysis showed a 1578 bp deletion in intron 8 of Bbs7 in BFMI mice. A CTCF-element is located inside this deletion. To investigate the functional effect of this deletion, it was introduced into B6N mice using CRISPR/Cas9. Two mice containing the target deletion were obtained (B6N Bbs7emI8∆1 and Bbs7emI8∆2) and were subsequently mated to BFMI and B6N to generate two families suitable for complementation. Inherited alleles were determined and body composition was measured by quantitative magnetic resonance. Evidence for a partial complementation (13.1–15.1%) of the jObes1 allele by the CRISPR/Cas9 modified B6N Bbs7emI8∆1 and Bbs7emI8∆2 alleles was found. Mice carrying the complementation alleles had a 23–27% higher fat-to-lean ratio compared to animals which have a B6N allele (P(Bbs7emI8∆1) = 4.25 × 10–7; P(Bbs7emI8∆2) = 3.17 × 10–5). Consistent with previous findings, the recessive effect of the BFMI allele was also seen for the B6N Bbs7emI8∆1 and Bbs7emI8∆2 alleles. However, the effect size of the B6N Bbs7emI8∆1 and Bbs7emI8∆2 alleles was smaller than the BFMI allele, and thus showed only a partial complementation. Findings suggest additional variants near Bbs7 in addition to or interacting with the deletion in intron 8.

Item Type: Article
Additional Information: Funding Information: FK and KM were supported by the Deutsche Forschungsgemeinschaft (DFG) (BR 1285/12). We thank Angelika Ackermann for her assistance in the lab, Ben Gerhardt, Maximilian Sprechert, and Erik Leipe for genotyping. Marion Bütow, Michael Modrow, and Jens Fuchs for taking care of the animals and measuring phenotypes.
Subjects: C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: John Coen
Date Deposited: 13 Sep 2022 08:37
Last Modified: 13 Sep 2022 08:45
URI: https://nrl.northumbria.ac.uk/id/eprint/50110

Actions (login required)

View Item View Item


Downloads per month over past year

View more statistics