Isolation, Identification and Pathogenic Effects of Trichoderma spp. from Auricularia auricula

Dang, Hui, Kong, Qianqian, Winchester, William, Wan, Xin, Lei, Yu, Zhang, Haisheng, Zhao, Yu, Liu, Xinyu, Xu, Bin, Zhang, Baoshan and Wang, Zhe (2022) Isolation, Identification and Pathogenic Effects of Trichoderma spp. from Auricularia auricula. Advanced Composites and Hybrid Materials. ISSN 2522-0128 (In Press)

[img] Text
Manuscript_1.pdf - Accepted Version
Restricted to Repository staff only until 10 October 2023.

Download (1MB) | Request a copy

Abstract

Auricularia auricula, one of the most important edible mushrooms, is affected heavily by Trichoderma. We collected the diseased samples from the main A.auricula cultivation regions to characterize the pathogen and study the effect of Trichoderma spp. on A.auricula species. We identified one Trichoderma species, T.pleuroticola, based on the internal transcribed spacer and morphology characteristics and two types of A.auricula strains, Heiwei 15 (HW 15) and Hei 29 (H 29) were tested in this work. The growth rate of T.pleuroticola was 3.26-3.52 times higher than that of A.auricula and advantageously competed for living space and nutrients. In confrontation culture, T.pleuroticola completely inhibited the mycelium growth of A.auricula and grew on it, resulting in a diverse impact on HW 15 and H 29. In addition, T.pleuroticola can produce metabolites with antibacterial activity. The inhibition rate of volatile metabolites to H-29 and HW 15 was 13.46 and 10.44, and the inhibition rate of nonvolatile metabolites to H-29 and HW 15 was 36.04 and 31.49, respectively. Further analysis showed that these antifungal activities inhibiting Auricularia auricula growth were mainly attributed to the organic compounds from T.pleuroticola, nonanal, tyrosine, beta-sitosterol, and wortmannin. In short, T. pleuroticola was a highly pathogenic fungi in the production of A.auricula.

Item Type: Article
Additional Information: Funding information: Funding This research was funded by the National Key Research and Development Projects during the 13th Five Year Plan (2019YFC1606702); and by the Key Research and Development Projects of Shaanxi Province (2019NY-132; 2019NY-139; 2021NY-147). ZW acknowledges the support from Oakland University.
Uncontrolled Keywords: Auricularia auricula, Trichoderma pleuroticola, isolation and identification, pathogenic effect
Subjects: C200 Botany
Department: Faculties > Engineering and Environment > Mechanical and Construction Engineering
Depositing User: John Coen
Date Deposited: 14 Oct 2022 11:21
Last Modified: 14 Oct 2022 11:30
URI: https://nrl.northumbria.ac.uk/id/eprint/50390

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics