Ultrahigh-sensitivity label-free singlemode- tapered no core-singlemode fiber immunosensor for Listeria monocytogenes detection

Chen, Ling, Leng, Yuan-Kui, Qiu, Shi, Liu, Bin, Liu, Juan, Wan, Sheng-Peng, Wu, Tao, Xu, Hengyi, Xiong, Yonghua, Yuan, Jinhui, Xin, Xiangjun, Wang, Danling, He, Xing-Dao and Wu, Qiang (2023) Ultrahigh-sensitivity label-free singlemode- tapered no core-singlemode fiber immunosensor for Listeria monocytogenes detection. Sensors and Actuators B: Chemical, 376 (Part A). p. 132930. ISSN 0925-4005

2022.10.24 SNB manuscript with table.pdf - Accepted Version
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Official URL: https://doi.org/10.1016/j.snb.2022.132930


A challenge for optical fiber biosensor is to achieve ultrahigh sensitivity with narrow full width at half maximum (FWHM) of the spectrum. To address this challenge, an ultrahigh-sensitivity microfiber interferometer fiber ring laser (FRL) biosensor is proposed and investigated for Listeria monocytogenes (L. monocytogenes) detection. The fiber biosensor is composed of a singlemode- tapered no core-singlemode (STNS) fiber configuration, which is functionalized with the anti-L. monocytogenes antibodies. An Erbium Doped Fiber Amplifier is applied to the sensor to excite laser and thus reduce the FWHM of the spectrum, which significantly improved the limit of detection (LoD). The proposed STNS FRL biosensor has excellent reproducibility, specificity and sensitivity for L. monocytogenes. The developed STNS FRL biosensor can directly detect L. monocytogenes cells with LoD as low as 1.0 cell/mL, indicating the capability for detecting single cell of L. monocytogenes. Real lettuce and milk samples have been tested and test result in lettuce and milk samples has deviations within ±30% from that of Phosphate-buffered saline (PBS) for L. monocytogenes concentrations vary from 101 to 103 cells/mL(g). The developed STNS FRL biosensor has ultrahigh sensitivity, good stability, reproducibility, and specificity, which has potential applications in diseases/medical diagnostics.

Item Type: Article
Additional Information: Funding information: This work was supported by the National Natural Science Foundation of China (NSFC) (62175097, 62065013, 11864025, 61865013); Natural Science Foundation of Jiangxi Province (20192ACB20031, 20192ACBL21051 and 20202ACBL202002), Key R&D Projects of the Ministry of Science and Technology of China (2018YFE0115700); Royal Society International Exchanges 2021 Round 2 (IES\R2\212135).
Uncontrolled Keywords: Fiber optic sensor, fiber ring laser, Listeria monocytogenes, foodborne pathogens.
Subjects: F100 Chemistry
H600 Electronic and Electrical Engineering
Department: Faculties > Engineering and Environment > Mathematics, Physics and Electrical Engineering
Depositing User: Elena Carlaw
Date Deposited: 02 Nov 2022 08:52
Last Modified: 02 Dec 2022 16:15
URI: https://nrl.northumbria.ac.uk/id/eprint/50499

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