McDonald, Tom, Martin, Philip, Patterson, Joseph, Smith, Darren, Giardiello, Marco, Marcello, Marco, See, Violaine, O'Reilly, Rachel, Owen, Andrew and Rannard, Steven (2012) Multicomponent organic nanoparticles for fluorescence studies in biological systems. Advanced Functional Materials, 22 (12). pp. 2469-2478. ISSN 1616-301X
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Abstract
The formation of dual-component organic nanoparticles by a modified emulsion-templated freeze-drying approach leads to aqueous nanosuspensions showing fluorescence (Förster) resonance energy transfer (FRET) from within a distribution of single nanoparticles. The combination of both FRET dyes within dual-component nanoparticles (<200 nm) allows the spatial and physical monitoring of the particles, as the FRET signal is lost on dissolution and breakdown of the nanoparticles. The monitoring of accumulation by Caco-2 cells and macrophages shows very limited internalization within the non-phagocytic cells. Conservation of FRET within the macrophages confirms extensive whole-particle internalization. The cellular permeability through Caco-2 monolayers is also assessed and movement of intact dual-component particles is observed, suggesting a mechanism for enhanced pharmacokinetics in vivo.
| Item Type: | Article |
|---|---|
| Additional Information: | This is the pre-peer reviewed version of the following article: McDonald, T. O., Martin, P., Patterson, J. P., Smith, D., Giardiello, M., Marcello, M., See, V., O'Reilly, R. K., Owen, A. and Rannard, S. (2012), Multicomponent Organic Nanoparticles for Fluorescence Studies in Biological Systems. Adv. Funct. Mater., 22: 2469–2478 which has been published in final form at http://dx.doi.org/10.1002/adfm.201103059 |
| Uncontrolled Keywords: | nanosuspensions, cellular uptake, fluorescence resonance energy transfer (FRET) |
| Subjects: | C100 Biology C700 Molecular Biology, Biophysics and Biochemistry |
| Department: | Faculties > Health and Life Sciences > Applied Sciences |
| Depositing User: | Ay Okpokam |
| Date Deposited: | 26 Mar 2012 09:11 |
| Last Modified: | 17 Dec 2023 12:47 |
| URI: | https://nrl.northumbria.ac.uk/id/eprint/5861 |
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