Temporal and quantitative profiles of growth factors and metalloproteinases in acute wound fluid after mastectomy

Baker, Elizabeth, Kumar, Senthil, Melling, Andrew, Whetter, Deborah and Leaper, David (2008) Temporal and quantitative profiles of growth factors and metalloproteinases in acute wound fluid after mastectomy. Wound Repair and Regeneration, 16 (1). pp. 95-101. ISSN 1067-1927

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Abstract

Seventy-three samples of acute wound fluid were collected from 47 patients during the first 3 postoperative days (POD) following mastectomy for cancer (n=47 on POD-1, n=19 on POD-2, and n=7 POD-3). Samples were analyzed by enzyme-linked immunosorbent assay for growth factor levels (epidermal [EGF], platelet-derived [PDGF], basic fibroblast [bFGF], transforming growth factor-?1 [TGF-?1], vascular endothelial [VEGF]), interleukin-6 (IL-6), matrix metalloproteinases (MMPs-2, -3, -9), and the tissue inhibitor of metalloproteinase 1 (TIMP-1). The levels of EGF, bFGF, PDGF, and interleukin-6 peaked on POD-1, with a significant decrease by POD-3, while total and active MMP-2, MMP-3, and tissue inhibitor of metalloproteinase 1 showed a progressive and significant increase from days 1 to 3. The wounds that later developed an infection (11%) were found to have a significantly lower PDGF and EGF on day 1 (PDGF, median 169 pg/mL [range, 86–2,595]) than the noninfected wounds (2,098 [17–66,506] p<0.05, Mann–Whitney U-test). Sixty-two percent patients developed a seroma and the levels of bFGF were significantly less in these patients (441 pg/mL [45–4,108]) than in those patients where there was no seroma (807 [245–3,133] p<0.05). The levels of certain growth factors in acute wound fluid may be important markers for wound outcomes.

Item Type:	Article
Subjects:	A300 Clinical Medicine
Department:	Faculties > Health and Life Sciences > Nursing, Midwifery and Health
Depositing User:	EPrint Services
Date Deposited:	09 Sep 2009 11:36
Last Modified:	31 Jul 2021 08:39
URI:	http://nrl.northumbria.ac.uk/id/eprint/684

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