Stewart, Christopher, Marrs, Emma, Magorrian, Sinead, Nelson, Andrew, Lanyon, Clare, Perry, John, Embleton, Nicholas, Cummings, Stephen and Berrington, Janet (2012) The preterm gut microbiota: changes associated with necrotizing enterocolitis and infection. Acta Paediatrica, 101 (11). pp. 1121-1127. ISSN 1651-2227
Full text not available from this repository. (Request a copy)Abstract
Aim: To describe gut colonization in preterm infants using standard culture and 16S gene rRNA profiling, exploring differences in healthy infants and those who developed NEC/late onset sepsis (LOS).
Methods: Ninety-nine stools from 38 infants of median 27-week gestation were cultured; 44 stools from 27 infants had their microbial profiles determined by 16S. Ordination analyses explored effects of patient variables on gut communities.
Results: Standard microbiological culture identified a mean of two organisms (range 0-7), DGGE 12 (range 3-18) per patient. Enterococcus faecalis and coagulase negative staphylococci (CONS) were most common by culture (40% and 39% of specimens). Meconium was not sterile. No fungi were cultured. Bacterial community structures in infants with NEC and LOS differed from healthy infants. Infants who developed NEC carried more CONS (45% vs 30%) and less Enterococcus faecalis (31% vs 57%). 16S identified Enterobacter and Staphylococcus presence associated with NEC/LOS, respectively.
Conclusions: Important differences were found in the gut microbiota of preterm infants who develop NEC/LOS. The relationship of these changes to current practices in neonatal intensive care requires further exploration.
Item Type: | Article |
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Uncontrolled Keywords: | culture, molecular, necrotizing enterocolitis, preterm, sepsis |
Subjects: | A300 Clinical Medicine C500 Microbiology |
Department: | Faculties > Health and Life Sciences > Applied Sciences |
Depositing User: | Stephen Cummings |
Date Deposited: | 21 Sep 2012 09:01 |
Last Modified: | 12 Oct 2019 18:27 |
URI: | http://nrl.northumbria.ac.uk/id/eprint/9014 |
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