Exposure to bile and gastric juice can impact the aerodigestive microbiome in people with cystic fibrosis

Al-Momani, Hafez, Perry, Audrey, Nelson, Andrew, Stewart, Christopher J., Jones, Rhys, Krishnan, Amaran, Robertson, Andrew, Bourke, Stephen, Doe, Simon, Cummings, Stephen, Anderson, Alan, Forrest, Tara, Forrest, Ian, Griffin, Michael, Wilcox, Matthew, Brodlie, Malcolm, Pearson, Jeffrey and Ward, Christopher (2022) Exposure to bile and gastric juice can impact the aerodigestive microbiome in people with cystic fibrosis. Scientific Reports, 12 (1). p. 11114. ISSN 2045-2322

s41598-022-15375-4.pdf - Published Version
Available under License Creative Commons Attribution 4.0.

Download (1MB) | Preview
Official URL: https://doi.org/10.1038/s41598-022-15375-4


Studies of microbiota reveal inter-relationships between the microbiomes of the gut and lungs. This relationship may influence the progression of lung disease, particularly in patients with cystic fibrosis (CF), who often experience extraoesophageal reflux (EOR). Despite identifying this relationship, it is not well characterised. Our hypothesis is that the gastric and lung microbiomes in CF are related, with the potential for aerodigestive pathophysiology. We evaluated gastric and sputum bacterial communities by culture and 16S rRNA gene sequencing in 13 CF patients. Impacts of varying levels of bile acids, pepsin and pH on patient isolates of Pseudomonas aeruginosa (Pa) were evaluated. Clonally related strains of Pa and NTM were identified in gastric and sputum samples from patients with symptoms of EOR. Bacterial diversity was more pronounced in sputa compared to gastric juice. Gastric and lung bile and pepsin levels were associated with Pa biofilm formation. Analysis of the aerodigestive microbiomes of CF patients with negative sputa indicates that the gut can be a reservoir of Pa and NTM. This combined with the CF patient's symptoms of reflux and potential aspiration, highlights the possibility of communication between microorganisms of the gut and the lungs. This phenomenon merits further research.

Item Type: Article
Additional Information: Funding Information: H Al-momani received financial support from the faculty of medicine at The Jordanian Hashemite University. C Ward, J Pearson, SM Griffin and Rhys Jones were supported by a UK Government Technology Strategy Board Knowledge Transfer Partnership (Certificate No. KTP008821), between Newcastle University and Newcastle upon Tyne Hospitals NHS Foundation Trust. C Ward was supported by the Medical Research Foundation Grant MRF-091-0001-RG-GARNE. And an unrestricted research grant from Boehringer Ingelheim. M Brodlie supported by a Medical Research Council Clinician Scientist fellowship. This research was supported by a Venture and Innovation Award from the CF Trust. Grant reference No. VIA 099.
Uncontrolled Keywords: Pseudomonas aeruginosa - genetics, Gastroesophageal Reflux - complications, Humans, Sputum - microbiology, Cystic Fibrosis - microbiology, RNA, Ribosomal, 16S - genetics, Microbiota - genetics, Bacteria, Pepsin A, Lung - microbiology, Gastric Juice - microbiology, Bile
Subjects: A300 Clinical Medicine
B400 Nutrition
B900 Others in Subjects allied to Medicine
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Rachel Branson
Date Deposited: 09 Aug 2022 15:14
Last Modified: 09 Aug 2022 15:15
URI: http://nrl.northumbria.ac.uk/id/eprint/49805

Actions (login required)

View Item View Item


Downloads per month over past year

View more statistics