Sterol 3β-glucosyltransferase biocatalysts with a range of selectivities, including selectivity for testosterone

Malik, Vatsala, Zhang, Meng, Dover, Lynn, Northen, Julian, Flinn, Anthony, Perry, Justin and Black, Gary (2013) Sterol 3β-glucosyltransferase biocatalysts with a range of selectivities, including selectivity for testosterone. Molecular BioSystems, 9 (11). pp. 2816-2822. ISSN 1742-206X

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Abstract

The main objectives of this work were to characterise a range of purified recombinant sterol 3β-glucosyltransferases and show that rational sampling of the diversity that exists within sterol 3β-glucosyltransferase sequence space can result in a range of enzyme selectivities. In our study the catalytically active domain of the Saccharomyces cerevisiae 3β-glucosyltransferase was used to mine putative sterol 3β-glucosyltransferases from the databases. Selected diverse sequences were expressed in and purified from Escherichia coli and shown to have different selectivities for the 3β-hydroxysteroids ergosterol and cholesterol. Surprisingly, three enzymes were also selective for testosterone, a 17β-hydroxysteroid. This study therefore reports for the first time sterol 3β-glucosyltransferases with selectivity for both 3β- and 17β-hydroxysteroids and is also the first report of recombinant 3β-glucosyltransferases with selectivity for steroids with a hydroxyl group at positions other than C-3. These enzymes could therefore find utility in the pharmaceutical industry for the green synthesis of a range of glycosylated compounds of medicinal interest.

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