Metabolic programming of a beige adipocyte phenotype by genistein

Aziz, Sadat, Wakeling, Luisa, Miwa, Satomi, Alberdi, Goiuri, Hesketh, John and Ford, Dianne (2017) Metabolic programming of a beige adipocyte phenotype by genistein. Molecular Nutrition & Food Research, 61 (2). p. 1600574. ISSN 1613-4125

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Promoting the development of brown or beige adipose tissue may protect against obesity and related metabolic features, and potentially underlies protective effects of genistein in mice.
Methods and results

We observed that application of genistein to 3T3-L1 adipocytes changed the lipid distribution from large droplets to a multilocular distribution, reduced mRNAs indicative of white adipocytes (ACC, Fasn, Fabp4, HSL, chemerin, and resistin) and increased mRNAs that are a characteristic feature of brown/beige adipocytes (CD-137 and UCP1). Transcripts with a role in adipocyte differentiation (Cebpβ, Pgc1α, Sirt1) peaked at different times after application of genistein. These responses were not affected by the estrogen receptor (ER) antagonist fulvestrant, revealing that this action of genistein is not through the classical ER pathway. The Sirt1 inhibitor Ex-527 curtailed the genistein-mediated increase in UCP1 and Cebpβ mRNA, revealing a role for Sirt1 in mediating the effect. Baseline oxygen consumption and the proportional contribution of proton leak to maximal respiratory capacity was greater for cells exposed to genistein, demonstrating greater mitochondrial uncoupling.

We conclude that genistein acts directly on adipocytes or on adipocyte progenitor cells to programme the cells metabolically to adopt features of beige adipocytes. Thus, this natural dietary agent may protect against obesity and related metabolic disease.

Item Type: Article
Uncontrolled Keywords: Brown adipose tissue, estrogen receptor, Genistein, SIRT1, White adipose tissue
Subjects: B400 Nutrition
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Becky Skoyles
Date Deposited: 09 Jan 2017 11:51
Last Modified: 01 Aug 2021 05:16

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