Modelling the role of microbial p-cresol in colorectal genotoxicity.

Al Hinai, Eiman Abdulla, Kullamethee, Piyarach, Rowland, Ian R., Swann, Jonathan, Walton, Gemma E. and Commane, Daniel M. (2019) Modelling the role of microbial p-cresol in colorectal genotoxicity. Gut microbes, 10 (3). pp. 398-411. ISSN 1949-0984

Preview	Text (Final published version) Modelling the role of microbial p cresol in colorectal genotoxicity.pdf - Published Version Available under License Creative Commons Attribution 4.0. Download (3MB) | Preview
Preview	Text (Advance online version) Modelling the role of microbial p cresol in colorectal genotoxicity.pdf - Published Version Available under License Creative Commons Attribution 4.0. Download (2MB) | Preview

Abstract

ackground: A greater understanding of mechanisms explaining the interactions between diet and the gut microbiota in colorectal cancer is desirable. Genotoxic microbial metabolites present in the colon may be implicated in carcinogenesis and potentially influenced by diet.

Aims: We hypothesised that microbial p-cresol is a colonic genotoxin and set out to model potential exposures in the colon and the effects of these exposures on colonic cells.

Methods: Batch culture fermentations with human faecal inoculate were used to determine the synthesis of p-cresol and other metabolites in response to various substrates. The fermentation supernatants were evaluated for genotoxicity and the independent effects of p-cresol on colonic cells were studied in vitro.

Results: In batch culture fermentation, supplementary protein increased the synthesis of phenols, indoles and p-cresol, whereas supplementary fructoligosaccharide (FOS) increased the synthesis of short chain fatty acids. The p-cresol was the greatest predictor of genotoxicity against colonocytes in the fermentation supernatants. Spiking fermentation supernatants with exogenous p-cresol further increased DNA damage, and independently p-cresol induced DNA damage in a dose-dependent manner against HT29 and Caco-2 cells and influenced cell cycle kinetics.

Conclusions: In the colon p-cresol may reach physiologically significant concentrations which contribute to genotoxic exposures in the intestinal lumen, p-cresol production may be attenuated by substrate, and therefore diet, making it a potential modifiable biomarker of genotoxicity in the colon.

Actions (login required)

View Item

Downloads