Immunochemical and Mass Spectrometric Analysis of Nε-(Carboxymethyl)lysine Content of AGE−BSA Systems Prepared with and without Selected Antiglycation Agents

Srey, Chou, Haughey, Simon, Connolly, Lisa, del Castillo, Maria Dolores, Elliott, Christopher and Ames, Jenny (2010) Immunochemical and Mass Spectrometric Analysis of Nε-(Carboxymethyl)lysine Content of AGE−BSA Systems Prepared with and without Selected Antiglycation Agents. Journal of Agricultural and Food Chemistry, 58 (22). pp. 11955-11961. ISSN 0021-8561

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Abstract

The present study was designed to compare surface plasmon resonance (SPR) biosensor, enzyme-linked immunosorbent assay (ELISA), and ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) methods for the analysis of N(epsilon)-(carboxymethyplysine (CML) in glucose bovine serum albumin (BSA) model systems and to investigate the possible inhibitory effect of selected compounds (a-tocopherol, ferulic acid, rutin, thiamin, thiamin monophosphate, and thiamin pyrophosphate) on CML formation. The reported levels of CML detected were dependent upon the method of analysis employed. The highest reported concentrations were obtained with the SPA biosensor, whereas the lowest were found by ELISA. However, a high correlation was observed between these two immunochemical procedures. CML concentrations were dependent upon the type and concentration of the candidate CML inhibitor. All inhibitory compounds investigated, with the exception of a-tocopherol, decreased the level of CML formation in the glucose BSA system.

Item Type:	Article
Uncontrolled Keywords:	N(epsilon)-(Carboxymethyl)lysine (CML),advanced glycation endproducts (AGEs),CML inhibitor, enzyme-linked immunosorbent assay (ELISA), surface plasmon resonance (SPR) biosensor, ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)
Subjects:	B400 Nutrition C900 Others in Biological Sciences
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	Ay Okpokam
Date Deposited:	13 Dec 2011 17:09
Last Modified:	12 Oct 2019 18:25
URI:	http://nrl.northumbria.ac.uk/id/eprint/4026

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