Large‐Scale Fabrication of 3D Scaffold‐Based Patterns of Microparticles and Breast Cancer Cells using Reusable Acoustofluidic Device

Nguyen, Tan Dai, Tran, Van-Thai, Pudasaini, Sanam, Gautam, Archana, Lee, Jia Min, Fu, Richard and Du, Hejun (2021) Large‐Scale Fabrication of 3D Scaffold‐Based Patterns of Microparticles and Breast Cancer Cells using Reusable Acoustofluidic Device. Advanced Engineering Materials, 23 (6). p. 2001377. ISSN 1438-1656

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Abstract

Spatial distribution of biological cells plays a key role in tissue engineering for reconstituting the cellular microenvironment, and recently acoustofluidics have been explored as a viable tool for controlling structures in tissue fabrication because of its good biocompatibility, low-power consumption, automation capability, nature of non-invasive and non-contact. Herein, we developed a reusable acoustofluidic device using surface acoustic waves for manipulating microparticles/cells to form a three-dimensional (3D) matrix pattern inside a scaffold-based hydrogel contained in a millimetric chamber. The 3D patterned and polymerized hydrogel construct can be easily and safely removed from the chamber using a proposed lifting technique, which prevent any physical damages or contaminations and promote the reusability of the chamber. The generated 3D patterns of microparticles and cells were numerically studied using finite element method, which was well validated by the experimental results. Our proposed acoustofluidic device is a useful tool for in vitro engineering three-dimensional scaffold-based artificial tissues for drug and toxicity testing and building organs-on-chip applications.

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