Li, Chong, Zhang, Jikai, Xie, Haiyu, Luo, Jingting, Fu, Chen, Tao, Ran, Li, Honglang and Fu, Yong Qing (2022) Highly Sensitive Love Mode Acoustic Wave Platform with SiO2 Wave-Guiding Layer and Gold Nanoparticles for Detection of Carcinoembryonic Antigens. Biosensors, 12 (7). p. 536. ISSN 2079-6374
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Biosensors accepted manuscript.v7-0717 - clear.pdf - Accepted Version Available under License Creative Commons Attribution 4.0. Download (896kB) | Preview |
Abstract
A highly sensitive and precise Love wave mode surface acoustic wave (SAW) immunosensor based on an ST-cut 90°X quartz substrate and an SiO2 wave-guiding layer was developed to detect cancer-related biomarkers of carcinoembryonic antigens (CEAs). A delay line structure of the SAW device with a resonant frequency of 196 MHz was designed/fabricated, and its surface was functionalized through CEA antibody immobilization. The CEA antibodies were bound with gold nanoparticles and CEA antibodies to form a sandwich structure, which significantly amplified the mass loading effect and enhanced the maximum responses by 30 times. The center frequency of the Love wave immunosensor showed a linear response as a function of the CEA concentration in the range of 0.2–5 ng/mL. It showed a limit of detection of 0.2 ng/mL, and its coefficient of determination was 0.983. The sensor also showed minimal interference from nonspecific adsorptions, thus demonstrating its promise for point-of-care applications for cancer biomarkers.
| Item Type: | Article |
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| Additional Information: | Funding information: This work was financially supported by the Research Project in Fundamental and Application Fields of Guangdong Province (Grant no. 2020A1515110561, 2019A1515111199), Research and Development Program of Guangdong Province (Grant no. 2020B0101040002), Key Research Program of Education Department for Guangdong Province (Grant no. 2020ZDZX2007), National Natural Science Foundation of China (NSFC Grant no. 12104320), Shenzhen Science & Technology Project (Grant no. RCBS20200714114918249, GJHZ20200731095803010), Engineering and Physical Sciences Research Council of UK (EPSRC EP/P018998/1), Special Interest Group for Acoustofluidics from UK Fluids Network (EP/N032861/1), International Exchange Grant (IEC/NSFC/201078) through the Royal Society and NSFC, and EPSRC NetworkPlus in Digitalised Surface Manufacturing (EP/S036180/1). |
| Uncontrolled Keywords: | love wave; biosensors; CEA; AuNPs |
| Subjects: | H800 Chemical, Process and Energy Engineering |
| Department: | Faculties > Engineering and Environment > Mathematics, Physics and Electrical Engineering |
| Depositing User: | Elena Carlaw |
| Date Deposited: | 18 Jul 2022 14:23 |
| Last Modified: | 19 Jul 2022 08:30 |
| URI: | http://nrl.northumbria.ac.uk/id/eprint/49568 |
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